Mass in the lateral cervical-thoracic region in a male Wistar rat. Adenocarcinoma of the mammary gland.
Mass in the lateral cervical-thoracic region in a male Wistar rat. Adenocarcinoma of the mammary gland.
复制标题
雄性 Wistar 大鼠颈胸外侧区域的肿块。
DOI：
10.1038/laban0909-288
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发表时间：
2009
期刊：
影响因子：
6.9
通讯作者：
Wells,MoniqueY
中科院分区：
文献类型：
--
作者：
Kalaiselvan,Ponnusamy;Vijayakumar,Subramaniyan;Hemalatha,Kuppusamy;Murkunde,YogeshkumarV;Herbert,RonaldA;Wells,MoniqueY
对来自国际生物技术和毒理学研究所繁殖群的WNIN品系（Wistar/NIN）37周龄雄性Wistar大鼠进行尸检，其颈胸区左侧有肿块病史。我们在标准实验室条件下饲养大鼠。室温为18-21 ℃，相对湿度为40-70%，光：暗循环为12小时：12小时。我们给予大鼠来自商业供应商的颗粒状饲料，并提供过滤的紫外线处理水供自由饮用。我们对那只老鼠进行了彻底的尸检。尸检时，在颈胸区左侧观察到一个直径约4 cm的坚硬、结节状、不动的皮下肿块（图1）。我们切开肿块，看到不规则的囊腔（图1），囊腔内含有浆液性血液。在检查的其他器官中未观察到异常。我们未发现我们实验室先前在9月龄雄性或雌性大鼠中报告的类似肉眼可见病变。将所有器官（包括颈胸肿块）固定在10%中性缓冲福尔马林中。对组织进行处理，石蜡包埋，切片为3-4 µm，并用苏木精和伊红染色，用于组织病理学评价。组织学上，肿块细胞密集，被厚囊包围，胶原带延伸到实质中（图2a）。它有一个膨胀的生长模式，细胞排列在小叶中，由精细的结缔组织基质支持。尸检时观察到的囊性空间被中性粒细胞浸润，并含有坏死细胞碎片（图2b）。虽然在某些区域细胞呈带状和岛状排列，但大部分肿块由固体、致密、均匀的细胞片组成（图2c），其特征是细胞质稀少、嗜两性和均一性B c a
A 37-week-old male Wistar rat of strain WNIN (Wistar/NIN) from the breeding colony of the International Institute of Biotechnology and Toxicology was presented for necropsy with the history of a mass in the left lateral aspect of the cervicothoracic region. We maintained the rat under standard laboratory conditions. The room temperature was 18–21 C, relative humidity was 40–70% and the light: dark cycle was 12-h: 12-h. We gave the rat a pelleted diet from a commercial supplier and provided filtered, ultraviolet-treated water ad libitum. We carried out a complete necropsy on the rat. At necropsy, a firm, nodular, immobile, subcutaneous mass measuring approximately 4 cm in diameter was observed at the left lateral aspect of the cervicothoracic region (Fig. 1). We incised the mass and saw irregular cystic spaces (Fig. 1) that contained serosanguineous fluid. No abnormalities were seen in the other organs examined. We were not aware of similar macroscopic lesions being reported in our laboratory previously, in 9-month-old rats, either male or female. All organs, including the cervicothoracic mass, were fixed in 10% neutral buffered formalin. Tissues were processed, embedded in paraffin, sectioned at 3–4 µm and stained with hematoxylin and eosin for histopathology evaluation. Histologically, the mass was densely cellular and surrounded by a thick capsule with bands of collagen extending into the parenchyma (Fig. 2a). It had an expansive growth pattern with cells arranged in lobules supported by fine connective tissue stroma. The cystic spaces seen at necropsy were infiltrated by neutrophils and contained necrotic cellular debris (Fig. 2b). Although cells were arranged in ribbons and islands in some areas, most of the mass was composed of solid, compact, uniform sheets of cells (Fig. 2c) characterized by scanty, amphophilic and homogenous cytoplasm b c a