ANTHER CULTURE OF TROPICAL JAPONICA X INDICA HYBRIDS OF RICE (ORYZA-SATIVA L)
ANTHER CULTURE OF TROPICAL JAPONICA X INDICA HYBRIDS OF RICE (ORYZA-SATIVA L)
复制标题
DOI：
10.1007/bf00041756
复制
发表时间：
1992-01-01
期刊：
影响因子：
1.9
通讯作者：
VERGARA, G
中科院分区：
文献类型：
--
作者：
GUIDERDONI, E;GALINATO, E;VERGARA, G
对9个籼粳杂种F_1进行了大规模花药培养，其中包括6个籼稻品种和3个热带粳稻品种。花药愈伤率平均为18.7%。小孢子愈伤组织产生绿色植株的平均频率为8.7%。白化病植株占芽形成愈伤组织的61%。对培养花药的第4周和第8周之间产生的愈伤组织的绿色和白化病植物再生能力的监测表明，在培养第6周后，白化病/绿色比率没有增加，转移的愈伤组织的芽形成能力缓慢下降。在4个杂种中，自发加倍单倍体（SDH）占再生绿色植株的46%。然而，高频率的部分不育的再生体中注意到132 SDH从杂种产生的植物。
Nine japonica x indica F1 hybrids of rice involving 6 indica and 3 japonica tropical varieties, were large scale anther cultured. The frequency of callusing anthers averaged 18.7%. The microspore-derived calli produced green plants with a mean frequency of 8.7%. Albino plants represented 61% of the shoot forming calli. Monitoring of the green and albino plant regenerating capabilities of calli arising between week 4 and week 8 of incubation of the anthers showed no increase of the albino/green ratio and a slow decrease of the shoot forming ability of the transferred calli after the sixth week of culture. Spontaneous doubled haploids (SDH) represented 46% of the regenerated green plants in 4 hybrids. However, a high frequency of partially sterile regenerants was noticed among 132 SDH plants generated from a hybrid.