Irritant and adjuvant effects of gaseous formaldehyde on the ovalbumin-induced hyperresponsiveness and inflammation in a rat model
Irritant and adjuvant effects of gaseous formaldehyde on the ovalbumin-induced hyperresponsiveness and inflammation in a rat model
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DOI：
10.3109/08958370902806159
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发表时间：
2009-12-01
影响因子：
2.1
通讯作者：
Yang, X.
中科院分区：
文献类型：
--
作者：
Qiao, Y.;Li, B.;Yang, X.
背景：甲醛（FA）是一种常见的室内空气污染物，可导致长期暴露的人发生哮喘。虽然FA和其他人造化学品有助于刺激哮喘在一般人群中，这种关系的潜在分子发病机制尚未得到很好的理解。（ii）卵清蛋白（OVA）;（iii）417 ppb的OVA + FA;（iv）2500 ppb的OVA + FA;和（v）2500 ppb的FA。中国目前和以前的职业接触限值分别为417 ppb和2500 ppb。在OVA免疫或盐水处理之前和期间，向动物施用气态FA 6小时/天。测量的结果包括原位肺功能分析，细胞因子测量，和组织学变化在大鼠lungs.Results：的气道反应性，肺组织学变化，肺白细胞介素-4分泌，和嗜酸性粒细胞浸润的OVA和FA暴露大鼠后，气体FA暴露417和2500 ppb的显着较高。虽然FA暴露单独没有引起显着的结构变化的气道，炎症细胞浸润的速率是相同的对照组，肺干扰素-γ水平显着升高暴露rats.Conclusions：FA可能是一种刺激物，以及作为一种佐剂的哮喘或哮喘样症状的发作。
Background: Formaldehyde (FA) is a common indoor air pollutant that can cause asthma in people experiencing long-term exposure. While FA and other man-made chemicals contribute to the stimulation of asthma in the general population, the underlying molecular pathogenesis of this relationship is not yet well understood.Objective: To explore FA as an irritant for the onset of asthma and as an adjuvant for the induction of allergy.Methods: In the present study, 40 Wistar rats in five experimental groups were exposed to: (i) saline; (ii) ovalbumin (OVA); (iii) OVA + FA at 417 ppb; (iv) OVA + FA at 2500 ppb; and (v) FA at 2500 ppb. Current and prior occupational exposure limits in China were established at 417 ppb and 2500 ppb, respectively. Gaseous FA was administrated to the animals for 6 h/day before and during OVA immunization or saline treatment. Measured outcomes included in situ lung function analysis, cytokine measurement, and histological changes in the rat lungs.Results: The airway reactivity, lung histological changes, pulmonary interleukin-4 secretion, and eosinophil infiltration in the OVA and FA exposed rats were significantly higher after gaseous FA exposures of 417 and 2500 ppb. While FA exposure alone did not induce significant structural changes to the airway, and the rate of inflammatory cell infiltration was the same as for the control group, pulmonary levels of interferon-gamma were significantly elevated in the exposed rats.Conclusions: FA may be an irritant as well as serve as an adjuvant for the onset of asthma or asthma-like symptoms.