Identification and molecular mapping of a gene in wheat conferring resistance to Mycosphaerella graminicola
Identification and molecular mapping of a gene in wheat conferring resistance to Mycosphaerella graminicola
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DOI：
10.1094/phyto.2003.93.9.1158
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发表时间：
2003-09-01
期刊：
影响因子：
3.2
通讯作者：
Goodwin, SB
中科院分区：
文献类型：
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作者：
Adhikari, TB;Anderson, JM;Goodwin, SB
小麦壳针孢叶斑病是由禾生球腔菌（Mycosphaerella graminicola）无性型引起的小麦重要病害。抗病育种是控制该病最有效的手段，可以通过分子标记的使用来促进抗病育种。然而，与大多数STB抗性基因连锁的分子标记尚不可用。进行本研究以测试标准小麦作图群体的亲本中的抗性并定位鉴定的任何抗性基因。该群体由130个F-10重组自交系（RILs）从人工合成的六倍体小麦W7984和cv之间的杂交。85. honor遗传分析表明，一个主效基因控制对M.在这个群体中的Graminicola这个假定的抗性基因现在被命名为Stb 8，并与扩增片段长度多态性（AFLP）和微卫星标记进行了映射。AFLP标记EcoRI-ACG/MseI-CAG 5与抗性基因在约5.3厘摩（cM）的距离处以排斥方式连锁。两个侧翼微卫星标记Xgwm 146和Xgwm 577与小麦7 B染色体长臂上的Stb 8基因连锁，距离分别为3.5和5.3cM。本研究所发现的微卫星标记有可能用于分子标记辅助选择育种，并用于Stb 8与其他抗稻瘟病基因的连锁。小麦中的禾生菌
Septoria tritici leaf blotch (STB), caused by the ascomycete Mycosphaerella graminicola (anamorph Septoria tritici), is an economically important disease of wheat. Breeding for resistance to STB is the most effective means to control this disease and can be facilitated through the use of molecular markers. However, molecular markers linked to most genes for resistance to STB are not yet available. This study was conducted to test for resistance in the parents of a standard wheat mapping population and to map any resistance genes identified. The population consisted of 130 F-10 recombinant-inbred lines (RILs) from a cross between the synthetic hexaploid wheat W7984 and cv. Opata 85. Genetic analysis indicated that a single major gene controls resistance to M. graminicola in this population. This putative resistance gene is now designated Stb8 and was mapped with respect to amplified fragment length polymorphism (AFLP) and microsatellite markers. An AFLP marker, EcoRI-ACG/MseI-CAG5, was linked in repulsion with the resistance gene at a distance of approximately 5.3 centimorgans (cM). Two flanking microsatellite markers, Xgwm146 and Xgwm577, were linked to the Stb8 gene on the long arm of wheat chromosome 7B at distances of 3.5 and 5.3 cM, respectively. The microsatellite markers identified in this study have potential for use in marker-assisted selection in breeding programs and for pyramiding of Stb8 with other genes for resistance to M. graminicola in wheat.