Isolation and Identification of Saccharomyces cerevisiae var boulardii and its Uses as a Probiotic (in vitro)
Isolation and Identification of Saccharomyces cerevisiae var boulardii and its Uses as a Probiotic (in vitro)
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酿酒酵母布拉氏变种的分离鉴定及其作为益生菌的用途（体外）
DOI：
10.33899/rjs.2014.86051
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Khanda O. Khidhr
中科院分区：
文献类型：
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作者：
Zainab M. Al Zubaidy;Khanda O. Khidhr
本研究包括酿酒酵母的分离和鉴定。从埃尔比勒市当地市场上可买到的新鲜和干燥的水果，如（无花果、葡萄、杏、荔枝、山竹）中提取boulardii。通过形态学、生理生化试验，共获得50株酵母菌。用微孔扩散法测定分离的酵母菌对致病菌沙门氏菌肠道亚种（鼠伤寒）的体外抗菌活性。大部分分离的酵母菌对供试菌均有抑菌活性，其中S.b32菌株的抑菌圈直径最大，为16 mm。并对不同浓度胆汁盐和胃酸的耐受能力进行了研究。从分离的酵母菌中筛选出10株具有最高抑菌活性的菌株，并对其生理特性（胆汁盐浓度和胃酸）进行了研究，它们是：S.b6、S.c1、S.b2、S.c36、S.b13、S.b28、S.b32、S.b22、S.b48和S.b5。结果表明，S.b32菌株对胆汁盐和胃酸的耐受性较强。
The study included isolation and identification of Saccharomyces cerevisiae var. boulardii from fresh and dried fruits such as (fig, grape, apricot, lychees, mangosteen) which were available in local markets in Erbil city. Fifty isolates of the yeast were obtained depending on morphological, physiological and biochemical tests. The antibacterial activity of the isolated yeasts against pathogenic bacteria Salmonella enteric sub spp.enterica (Typhimurium) in vitro was determined by Well's Diffusion method. Most of the isolated yeasts showed antibacterial activity against tested bacterium, the highest diameter of inhibition zone was (16 mm) for isolates (S.b32). The ability to tolerate different concentrations of bile salt and gastric acids were also studied. Ten strains of the isolated yeast had the highest inhibitory activity and were used to study physiological properties (bile salt concentration and gastric acidity) which were (S.b6, S.c1, S.b2, S.c36, S.b13, S.b28, S.b32, S.b22, S.b48, and S.b5). The result showed that the isolate (S.b32) was more resistant than other strains when exposed to bile salt and gastric acids.