Protection of bleomycin-induced fibrosis and inflammation by taurine.
Protection of bleomycin-induced fibrosis and inflammation by taurine.
复制标题
DOI：
10.1016/j.intimp.2009.04.003
复制
发表时间：
2009-07
影响因子：
5.6
通讯作者：
G. Schuller-Levis;R. Gordon;Chuanhua Wang;Seung-Yong Park;E. Park
中科院分区：
文献类型：
--
作者：
G. Schuller-Levis;R. Gordon;Chuanhua Wang;Seung-Yong Park;E. Park
牛磺酸已被证明可以防止由各种氧化剂（包括臭氧、二氧化氮、胺碘酮和百草枯）诱导的肺损伤，并与烟酸联合使用可以防止博来霉素诱导的肺损伤。在本研究中，在博来霉素滴注之前，用含5%牛磺酸的饮用水对Spraque-Dawley大鼠进行10天处理。与未处理的大鼠（BW）相比，用牛磺酸（BT）预处理的大鼠中不存在纤维化，沿着较少的炎性浸润。与BW组相比，BT组支气管肺泡灌洗液中的PMN数量显著减少，羟脯氨酸水平降低。通过免疫组化染色，诱导型一氧化氮合酶在博莱霉素治疗的大鼠的肺中是明显的，并且当大鼠用牛磺酸治疗时是最小的。通过免疫组织化学染色测量，牛磺酸处理和未处理大鼠的肺中均存在肿瘤坏死因子-α（TNF-α），但BW组中的含量高于BT组。此外，与BW组相比，BT组通过EM免疫金染色检测到ICAM呈递减少。这些数据表明，在博莱霉素滴注前，在饮用水中加入5%牛磺酸预处理的大鼠可避免纤维化、炎性浸润以及一氧化氮和TNF-α的产生，这是博莱霉素肺损伤的标志。
Taurine has been shown to protect against lung injury induced by various oxidants including ozone, nitrogen dioxide, amiodarone, and paraquat and to protect against bleomycin-induced lung injury in combination with niacin. In this study, Spraque-Dawley rats were treated with 5% taurine in the drinking water for 10 days prior to bleomycin instillation. Fibrosis in the rats pretreated with taurine (BT) was absent, along with fewer inflammatory infiltrates compared to the untreated rats (BW). A significant decrease in the number of PMNs and a decrease in hydroxyproline levels were found in the bronchoalveolar lavage fluid in the BT group compared to the BW group. By immunohistochemical staining, inducible nitric oxide synthase was evident in the lungs of bleomycin-treated rats, and minimal when rats were treated with taurine. Tumor necrosis factor-alpha (TNF-α) as measured by immunohistochemical staining, was present in lungs of both taurine-treated and untreated rats, but was more abundant in the BW group compared to the BT group. In addition, decreased ICAM presentation was detected by EM immunogold staining in the BT group compared to the BW group. These data demonstrate that rats pretreated with 5% taurine in their drinking water prior to bleomycin instillation are protected from fibrosis, inflammatory infiltrates, as well as nitric oxide and TNF-α production, which are hallmarks of bleomycin lung injury.