Requirement for the thymus in αβ T lymphocyte lineage commitment
Requirement for the thymus in αβ T lymphocyte lineage commitment
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DOI：
10.1016/s1074-7613(00)80601-9
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发表时间：
1998-08-01
期刊：
影响因子：
32.4
通讯作者：
Zúñiga-Pflücker, JC
中科院分区：
文献类型：
--
作者：
Carlyle, JR;Zúñiga-Pflücker, JC
我们最近发现了一个胎儿胸腺发育阶段(NK1.1(+)/CD117(Lo))，这是一个致力于T/NK祖细胞的阶段。我们现在报告在小鼠胎血和脾中存在表型和功能相同的T/NK祖细胞，但在胎肝中不存在。这些前体细胞与以前描述的胎儿血液“前胸腺细胞”(CD90(+)/CD117(Lo))几乎没有区别，只是它们表达NK1.1，缺乏与T谱系承诺相关的标记，维持着一个胚系TCRβ基因座，并能同时产生T和NK细胞。裸鼠体内存在NK1.1(+)/CD90(+)/CD117(Lo)的胎血前体细胞。这些结果表明T/NK谱系承诺途径是胸腺非依赖性的。相反，完全致力于α-βT血统并不是胸腺定植之前的事。
We recently identified a fetal thymic developmental stage (NK1.1(+)/CD117(lo)) that characterizes committed T/NK progenitors. We now report the existence of phenotypically and functionally identical T/NK progenitors in mouse fetal blood and spleen but not in fetal liver. These precursors are indistinguishable from previously characterized fetal blood "prothymocytes" (CD90(+)/CD117(lo)), with the exception that they express NK1.1, lack markers associated with T lineage commitment, maintain a germline TCR beta locus, and can give rise to both T and NK cells. Moreover, NK1.1(+)/CD90(+)/CD117(lo) fetal blood precursors are present in athymic nude mice. These results suggest that the T/NK lineage commitment pathway is thymus-independent. In contrast, full commitment to the alpha beta T lineage does not precede thymus colonization.