Raman spectroscopy of DNA packaging in individual human sperm cells distinguishes normal from abnormal cells
Raman spectroscopy of DNA packaging in individual human sperm cells distinguishes normal from abnormal cells
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DOI：
10.1002/jbio.200910012
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发表时间：
2009-05-01
影响因子：
2.8
通讯作者：
Balhorn, Rod
中科院分区：
文献类型：
--
作者：
Huser, Thomas;Orme, Christine A.;Balhorn, Rod
健康的男性会产生精子，其中约25%-40%的精子头部形状不正常。精子头部形态异常的精子百分率的增加与配偶不育有关，对混合精子的生化研究表明，形状异常的精子可能含有在精子细胞发育过程中未被鱼精蛋白正确重新包装的DNA。我们使用显微拉曼光谱从单个人类精子细胞获得拉曼光谱，并研究了精子染色质拉曼光谱的差异如何与细胞形状相关。我们发现，单个精子细胞的拉曼光谱包含可用于评估每个细胞的DNA包装效率的振动标记模式。从形状正常的精子细胞获得的拉曼光谱提供了证据，证明这些精子中的DNA被非常有效地包装。然而，我们发现，对于形状正常和异常的精子细胞，每个细胞的相对蛋白质含量和DNA包装效率分布在相对较宽的范围内。这些发现表明，单细胞拉曼光谱应该是评估体外受精精子细胞质量的有价值的工具。(C)2009年，威利-VCH Verlag GmbH&Co.KGaA，Weinheim
Healthy human males produce sperm cells of which about 25-40% have abnormal head shapes. Increases in the percentage of sperm exhibiting aberrant sperm head morphologies have been correlated with mate infertility, and biochemical studies of pooled sperm have suggested that sperm with abnormal shape may contain DNA that has not been properly repackaged by protamine during spermatid development. We have used micro-Raman spectroscopy to obtain Raman spectra from individual human sperm cells and examined how differences in the Raman spectra of sperm chromatin correlate with cell shape. We show that Raman spectra of individual sperm cells contain vibrational marker modes that can be used to assess the efficiency of DNA-packaging for each cell. Raman spectra obtained from sperm cells with normal shape provide evidence that DNA in these sperm is very efficiently packaged. We find, however, that the relative protein content per cell and DNA packaging efficiencies are distributed over a relatively wide range for sperm cells with both normal and abnormal shape. These findings indicate that single cell Raman spectroscopy should be a valuable tool in assessing the quality of sperm cells for in-vitro fertilization. (C) 2009 by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim