Cytoarchitectural reorganization of rabbit colonic goblet cells during baseline secretion.
Cytoarchitectural reorganization of rabbit colonic goblet cells during baseline secretion.
复制标题
基线分泌期间兔结肠杯状细胞的细胞结构重组。
DOI：
10.1002/aja.1001890408
复制
发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Specian,RD
中科院分区：
文献类型：
--
作者：
Radwan,KA;Oliver,MG;Specian,RD
Light and electron microscopy were coupled with point counting methods to quantitate shape and volume changes of goblet cells during their migration and maturation from the base of the crypt to the colonic surface epithelium in the rabbit. After differentiation, goblet cells attain a broad pyramidal configuration in the basal third of the crypt. The cells elongate and dramatically decrease in volume as they move into the surface epithelium. The distributions and volume fractions of organelles were found to vary considerably, depending on the location of the goblet cell in the epithelium. Mucin granules are initially synthesized throughout the cytoplasm, but become increasingly concentrated as the cell matures. Organelles involved in synthesis such as the Golgi apparatus and rough endoplasmic reticulum (RER) similarly attain a more concentrated arrangement as the cell moves up in the crypt. The mean cell volume decreases from 1,228.8 μm3for cells in the basal third of the crypt to 541.3 μm3for goblet cells on the surface. Most organelles decrease in proportion to this decrease, although a disproportionately large decrease in the RER was measured. When actual subcellular volumes are calculated, a net decrease in several subcellular compartments is detected. This loss of granules and organelles is accomplished by the continual synthesis and secretion of mucin granules. Cytoplasm and organelles become entrapped in the upward movement of granules towards the cell apex, become irretrievably isolated, and are sloughed into the crypt lumen. This process accounts for the decrease in cell volume and contributes to the altered cytoarchitecture of the cell.
