Luminal fructose modulates fructose transport and GLUT-5 expression in small intestine of weaning rats.
Luminal fructose modulates fructose transport and GLUT-5 expression in small intestine of weaning rats.
复制标题
腔内果糖调节断奶大鼠小肠中的果糖转运和 GLUT-5 表达。
DOI：
10.1152/ajpgi.1998.274.2.g232
复制
发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Ferraris,RP
中科院分区：
文献类型：
--
作者：
Shu,R;David,ES;Ferraris,RP
在新生大鼠中，早熟地引入膳食果糖可显着提高早期断奶期间的刷状缘果糖转运率和 GLUT-5 mRNA 水平。在这项研究中，与接受 Thiry-Vella 手术并食用高果糖 (HF) 饮食的断奶幼犬相比，吻合肠中的这些比率和水平高出两倍多。在喂食无果糖 (NF) 饮食的 Thiry-Vella 幼崽中，吻合肠中的摄取率和 mRNA 水平非常低，与旁路循环中的相似。在假手术的同窝仔鼠中，与 Thiry-Vella 幼仔中吻合和旁路环相对应的肠道区域之间的转运率和 mRNA 水平相似，并且饲喂 HF 的幼仔比饲喂 NF 饮食的幼仔高两到三倍。相反，刷状缘葡萄糖转运速率以及 SGLT-1 和 GLUT-2 mRNA 水平与饮食无关，并且在绕道区域和吻合区域之间相似。 GLUT-5 表达的变化不遵循明显的昼夜节律。当幼仔在饥饿 12 小时后喂食 HF 饮食以排空肠腔时，果糖转运率随着喂养时间的延长而增加，并在喂养后 12-24 小时达到稳定水平；相反，GLUT-5 mRNA水平在食糜到达空肠后4小时内达到最高，然后逐渐下降，24小时后恢复到基线水平。在饲喂 NF 饮食的同窝小鼠中，mRNA 水平和摄取率均与饲喂持续时间无关。断奶幼犬中的管腔信号（而非内分泌信号）调节 GLUT-5 的表达。
In neonatal rats, precocious introduction of dietary fructose significantly enhances brush-border fructose transport rates and GLUT-5 mRNA levels during early weaning. In this study, these rates and levels were more than two times higher in the anastomosed intestine compared with those in the bypassed loop of weaning pups that underwent Thiry-Vella surgery and consumed high-fructose (HF) diets. In Thiry-Vella pups fed fructose-free (NF) diets, uptake rates and mRNA levels in the anastomosed intestine were very low and similar to those in the bypassed loop. In sham-operated littermates, transport rates and mRNA levels were similar between intestinal regions that corresponded to anastomosed and bypassed loops in Thiry-Vella pups and were two to three times greater in pups fed HF than in those fed NF diet. In contrast, rates of brush-border glucose transport and levels of SGLT-1 and of GLUT-2 mRNA were independent of diet and were similar between bypassed and anastomosed regions. Changes in GLUT-5 expression did not follow a distinct diurnal rhythm. When pups were fed HF diet after 12 h of starvation to empty the intestinal lumen, fructose transport rates increased with feeding duration and reached a plateau 12–24 h after feeding; in contrast, GLUT-5 mRNA levels were highest within 4 h after arrival of chyme in the jejunum and then decreased gradually and returned to baseline levels 24 h later. In littermates fed NF diet, mRNA levels and uptake rates were each independent of feeding duration. Luminal, and not endocrine, signals regulate GLUT-5 expression in weaning pups.