MAT-ALPHA-1 PROTEIN, A YEAST TRANSCRIPTION ACTIVATOR, BINDS SYNERGISTICALLY WITH A 2ND PROTEIN TO A SET OF CELL-TYPE-SPECIFIC GENES
MAT-ALPHA-1 PROTEIN, A YEAST TRANSCRIPTION ACTIVATOR, BINDS SYNERGISTICALLY WITH A 2ND PROTEIN TO A SET OF CELL-TYPE-SPECIFIC GENES
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DOI：
10.1016/0092-8674(87)90326-6
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发表时间：
1987-08-28
期刊：
影响因子：
64.5
通讯作者：
SPRAGUE, GF
中科院分区：
文献类型：
--
作者：
BENDER, A;SPRAGUE, GF
我们通过电泳迁移率变化和DNAase 1足迹分析表明，酵母α1的产物α1。交配型基因座与三个已知的α-特异基因的控制区内的同源序列结合。结合需要α1和第二种酵母蛋白(称为PrTf)，该蛋白存在于所有三种细胞类型(α、α和α/α)中；两种蛋白都不能单独结合。使用合成寡核苷酸的结合和竞争实验表明，PRTF只找到在α特异基因上发现的部分同源性，并暗示α1与其余基因结合。我们的结果表明，α1通过为PRTF创建结合位点而使基因表达具有α特异性。类似的实验导致了这样一种想法，即PRTF也在a-特异性基因的转录中发挥作用。也许a-特异性是通过α2阻断PRTF结合位点而实现的，α2是由α编码的负调控因子。交配型基因座。
We show by electrophoresis mobility shift and by DNAase 1 footprinting assays that the .alpha.1 product of the yeast .alpha. mating-type locus binds to homologous sequences within the control regions of the three known .alpha.-specific genes. Binding requires both .alpha.1 and a second yeast protein(s) (called PRTF) that is present in all three cell types (a, .alpha., and a/.alpha.); neither protein binds alone. Binding and competition experiments using synthetic oligonucleotides indicate that PRTF finds to only part of the homology found at .alpha.-specific genes and imply that .alpha.1 binds to the remainder. Our results suggest that .alpha.1 renders gene expression .alpha.-specific by creating a binding site for PRTF. Similar experiments lead to the idea that PRTF also plays a role in transcription of a-specific genes. Perhaps a-specificity is achieved through the occlusion of the PRTF binding site by .alpha.2, the negative regulator encoded by the .alpha. mating-type locus.