Generation of a whole-brain atlas for the cholinergic system and mesoscopic projectome analysis of basal forebrain cholinergic neurons
Generation of a whole-brain atlas for the cholinergic system and mesoscopic projectome analysis of basal forebrain cholinergic neurons
复制标题
胆碱能系统全脑图谱的生成和基底前脑胆碱能神经元的介观投影组分析。
DOI：
10.1073/pnas.1703601115
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发表时间：
2018-01-09
影响因子：
11.1
通讯作者：
Qiu, Zilong
中科院分区：
文献类型：
--
作者：
Li, Xiangning;Yu, Bin;Qiu, Zilong
大脑中的胆碱能系统通过调节神经元活动，在调节感觉和运动功能以及认知行为方面起着至关重要的作用。要了解胆碱能系统的组织结构，需要一张完整的胆碱能神经元及其在整个大脑中单个神经元水平上的轴突分支的地图。在这里，我们报告了一个全面的全脑图谱胆碱能系统起源于小鼠大脑的不同皮质和皮质下区域。利用基因标记的胆碱能神经元，结合2微米分辨率的全脑重建光学图像，我们获得了22个脑区胆碱能神经元的数量和胞体体积的定量。此外，通过以1-PM分辨率重建来自基底前脑某一分区的单个荧光标记神经元的完整轴突，我们发现它们向前脑和中脑的投射显示出相同投影区内具有明显投射特异性和不同轴突分布的神经元亚群。这些结果表明，在大脑中存在不同亚型的胆碱能神经元，它们具有不同的调节功能，并说明了在中观水平上完全重建神经元分布和轴突投射的有用性。
The cholinergic system in the brain plays crucial roles in regulating sensory and motor functions as well as cognitive behaviors by modulating neuronal activity. Understanding the organization of the cholinergic system requires a complete map of cholinergic neurons and their axon arborizations throughout the entire brain at the level of single neurons. Here, we report a comprehensive whole-brain atlas of the cholinergic system originating from various cortical and subcortical regions of the mouse brain. Using genetically labeled cholinergic neurons together with whole-brain reconstruction of optical images at 2-mu m resolution, we obtained quantification of the number and soma volume of cholinergic neurons in 22 brain areas. Furthermore, by reconstructing the complete axonal arbors of fluorescently labeled single neurons from a subregion of the basal forebrain at 1-pm resolution, we found that their projections to the forebrain and midbrain showed neuronal subgroups with distinct projection specificity and diverse arbor distribution within the same projection area. These results suggest the existence of distinct subtypes of cholinergic neurons that serve different regulatory functions in the brain and illustrate the usefulness of complete reconstruction of neuronal distribution and axon projections at the mesoscopic level.