Usefulness of an inexpensive, Paracheck® test in detecting asymptomatic infectious reservoir of Plasmodium falciparum during dry season in an inaccessible terrain in central India
Usefulness of an inexpensive, Paracheck® test in detecting asymptomatic infectious reservoir of Plasmodium falciparum during dry season in an inaccessible terrain in central India
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DOI：
10.1053/jinf.2002.1055
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发表时间：
2002-10-01
影响因子：
28.2
通讯作者：
Sharma, VP
中科院分区：
文献类型：
--
作者：
Singh, N;Saxena, A;Sharma, VP
目的：评价一种新的国产快速诊断试验Paracheck PF(R)在印度中部曼德拉地区偏远森林村庄无症状儿童恶性疟原虫检测中的应用，以确定快速试验的敏感性和特异性下限。方法：采集手指抽血制备血涂片，用Paracheck试验进行检测。血液涂片是由一名经验丰富的技术人员阅读的，他对Paracheck的结果视而不见。以显微镜为金标准，计算特异度、敏感度、准确度和预测值。结果：新诊断试验的灵敏度为94%，特异度为89%。阳性预测值为71%，阴性预测值为98%。J指数为0.83%。结论：该快速检测方法简便易行，现场检测结果可靠。对这一新的廉价测试进行的实地评估(0.65美元/测试)表明，它可以作为疟疾管理的流行病学工具，特别是在显微镜操作不可行的地区，以实现减少疟疾倡议的目标。(C)2002年英国感染学会。爱思唯尔科学有限公司出版。版权所有。
Objectives: The performance of a new indigenous rapid diagnostic test, Paracheck Pf(R) was evaluated in detection of Plasmodium falciparum in asymptomatic children in remote forest villages of Mandla district, central India to determine the lower limits of sensitivity and specificity of rapid test.Methods: A finger prick blood sample was collected to prepare blood smear and for testing with the Paracheck test. The blood smears were read by an experienced technician blinded to the Paracheck results. The figures for specificity, sensitivity, accuracy and predictive values were calculated using microscopy as gold standard.Results: The new diagnostic test had a sensitivity of 94% and a specificity of 89%. The positive and negative predictive values were 71% and 98%, respectively. The J-index was 0.83%.Conclusion: The rapid test was found to be very easy to perform and the result could be read reliably by field workers. The field evaluation with this new inexpensive test, ($0.65/test) indicates that it could be used as an epidemiological tool in the management of malaria particularly in areas where microscopy is not operationally feasible to attain the goal of the roll back malaria initiative. (C) 2002 The British Infection Society. Published by Elsevier Science Ltd. All rights reserved.