Genetic analyses of the in vivo function of LolA, a periplasmic chaperone involved in the outer membrane localization of Escherichia coli lipoproteins
Genetic analyses of the in vivo function of LolA, a periplasmic chaperone involved in the outer membrane localization of Escherichia coli lipoproteins
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DOI：
10.1016/s0014-5793(98)01334-9
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发表时间：
1998-11-13
期刊：
影响因子：
3.5
通讯作者：
Tokuda, H
中科院分区：
文献类型：
--
作者：
Tajima, T;Yokota, N;Tokuda, H
大肠杆菌的主要外膜脂蛋白（Lpp）在定位于外膜中之前作为与LolA（周质伴侣）的复合物从细胞质膜释放到周质中。为了确定LolA是否通常参与体内脂蛋白的外膜定位，操作染色体lolA基因以便由inc启动子-操纵子控制。LolA的缺失导致严重的生长缺陷，并损害了Lpp和另一种外膜脂蛋白Lpp的外膜定位。尽管LolA缺失没有立即阻止缺乏Lpp的细胞的生长，但染色体lolA基因的破坏对lpp（-）菌株是致命的，表明LolA通常是脂蛋白外膜定位所需的，因此无论Lpp存在与否都是必要的，（C）1998欧洲生物化学学会联合会。
The major outer membrane lipoprotein (Lpp) of Escherichia coli is released from the cytoplasmic membrane into the periplasm as a complex with LolA, a periplasmic chaperone, prior to the localization in the outer membrane. To determine whether or not LolA is generally involved in the outer membrane localization of lipoproteins in vivo, the chromosomal lolA gene was manipulated so as to be controlled by the inc promoter-operator. Depletion of LolA caused a severe growth defect, and impaired the outer membrane localization of Lpp and Pal, another outer membrane lipoprotein, Although LolA depletion did not immediately arrest the growth of cells lacking Lpp, disruption of the chromosomal lolA gene was lethal to the lpp(-) strain, indicating that LolA is generally required for the outer membrane localization of lipoproteins, and therefore essential irrespective of the presence or absence of Lpp, (C) 1998 Federation of European Biochemical Societies.