Uncovering circuit mechanisms of current sinks and sources with biophysical simulations of primary visual cortex.
Uncovering circuit mechanisms of current sinks and sources with biophysical simulations of primary visual cortex.
复制标题
DOI：
10.7554/elife.87169
复制
发表时间：
2023-07-24
期刊：
影响因子：
7.7
通讯作者：
Arkhipov A
中科院分区：
文献类型：
--
作者：
Rimehaug AE;Stasik AJ;Hagen E;Billeh YN;Siegle JH;Dai K;Olsen SR;Koch C;Einevoll GT;Arkhipov A
局部场电位（LFP）记录反映了脑组织中电流源密度（CSD）的动态变化。突触、细胞和电路对电流汇和电流源的贡献还不清楚。我们使用公开的Neuropixels记录和基于模拟属于17种细胞类型的> 50，000个神经元的Hodgkin-Huxley动力学的详细电路模型来研究小鼠初级视觉皮层中的这些。该模型同时捕获尖峰和CSD响应，并表现出双向解离：通过调整突触权重改变放电率，对CSD模式产生轻微影响，通过调整树突上的突触位置改变CSD，对放电率产生轻微影响。我们描述了丘脑皮层的输入和经常性的连接雕塑特定的汇和源早期的视觉反应，而皮质反馈至关重要地改变他们在后期阶段。这些结果建立了宏观脑测量（LFP/CSD）和微观生物药理学为基础的神经元动力学的理解之间的定量联系，并表明CSD分析提供了强大的约束建模超出那些考虑尖峰。
Local field potential (LFP) recordings reflect the dynamics of the current source density (CSD) in brain tissue. The synaptic, cellular, and circuit contributions to current sinks and sources are ill-understood. We investigated these in mouse primary visual cortex using public Neuropixels recordings and a detailed circuit model based on simulating the Hodgkin–Huxley dynamics of >50,000 neurons belonging to 17 cell types. The model simultaneously captured spiking and CSD responses and demonstrated a two-way dissociation: firing rates are altered with minor effects on the CSD pattern by adjusting synaptic weights, and CSD is altered with minor effects on firing rates by adjusting synaptic placement on the dendrites. We describe how thalamocortical inputs and recurrent connections sculpt specific sinks and sources early in the visual response, whereas cortical feedback crucially alters them in later stages. These results establish quantitative links between macroscopic brain measurements (LFP/CSD) and microscopic biophysics-based understanding of neuron dynamics and show that CSD analysis provides powerful constraints for modeling beyond those from considering spikes.