A cytosolic class I small heat shock protein, RcHSP17.8, of Rosa chinensis confers resistance to a variety of stresses to Escherichia coli, yeast and Arabidopsis thaliana
A cytosolic class I small heat shock protein, RcHSP17.8, of Rosa chinensis confers resistance to a variety of stresses to Escherichia coli, yeast and Arabidopsis thaliana
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DOI：
10.1111/j.1365-3040.2009.01987.x
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发表时间：
2009-08-01
影响因子：
7.3
通讯作者：
Ming, Feng
中科院分区：
文献类型：
--
作者：
Jiang, Changhua;Xu, Jianyao;Ming, Feng
在高等植物的热激蛋白中，小热激蛋白（sHSP）家族的热激蛋白在功能上是最缺乏研究的。为了提高我们对sHSPs的理解，我们对月季中的RcHSP17.8进行了表征。序列比对和系统发育分析表明这是一个胞质I类sHSP。RcHSP 17.8在R.热、冷、盐、干旱、渗透胁迫和氧化胁迫均能诱导小白菜的愈伤组织分化。重组RcHSP 17.8在大肠杆菌和酵母中过表达，以研究其在应激条件下的可能功能。重组大肠与对照培养物相比，积累RcHSP17.8的大肠杆菌和酵母细胞在热、盐和氧化应激条件下表现出更高的活力。我们还产生了组成型表达RcHSP 17.8的转基因拟南芥。这些植物表现出对热、盐、渗透和干旱胁迫的耐受性增强。这些结果表明，R. chinensis胞质I类sHSP（RcHSP 17.8）不仅具有抗E.大肠杆菌和酵母菌，而且也对在各种不利环境条件下生长的植物有影响。
Among the heat shock proteins (HSPs) of higher plants, those belonging to the small HSP (sHSP) family remain the least characterized in functional terms. To improve our understanding of sHSPs, we have characterized RcHSP17.8 from Rosa chinensis. Sequence alignments and phylogenetic analysis reveal this to be a cytosolic class I sHSP. RcHSP17.8 expression in R. chinensis was induced by heat, cold, salt, drought, osmotic and oxidative stresses. Recombinant RcHSP17.8 was overexpressed in Escherichia coli and yeast to study its possible function under stress conditions. The recombinant E. coli and yeast cells that accumulated RcHSP17.8 showed improved viability under thermal, salt and oxidative stress conditions compared with control cultures. We also produced transgenic Arabidopsis thaliana that constitutively expressed RcHSP17.8. These plants exhibited increased tolerance to heat, salt, osmotic and drought stresses. These results suggest that R. chinensis cytosolic class I sHSP (RcHSP17.8) has the ability to confer stress resistance not only to E. coli and yeast but also to plants grown under a wide variety of unfavorable environmental conditions.