Recent advances on the GAP promoter derived expression system of Pichia pastoris
Recent advances on the GAP promoter derived expression system of Pichia pastoris
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DOI：
10.1007/s11033-008-9359-4
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发表时间：
2009-07-01
影响因子：
2.8
通讯作者：
Tu, Fa-zhi
中科院分区：
文献类型：
--
作者：
Zhang, Ai-Lian;Luo, Jin-Xian;Tu, Fa-zhi
巴斯德毕赤酵母（Pichia pastoris）是一种高效表达和分泌外源蛋白的宿主，其最重要的特征是醇氧化酶I（AOX 1）基因启动子的存在。AOX 1启动子（pAOX 1）已被用于在巴斯德毕赤酵母中表达外源基因并产生多种重组蛋白。然而，已经做出了一些努力来开发pAOX 1的新的替代启动子以避免使用甲醇。甘油醛-3-磷酸脱氢酶启动子（pGAP）已被用于许多异源蛋白的组成型表达。基于pGAP的表达系统更适合于大规模生产，因为消除了与大体积甲醇的储存和输送相关的危险和成本。本文就该表达系统的一些重要进展和特点作一综述。
Pichia pastoris is an efficient host for the expression and secretion of heterologous proteins and the most important feature of P. pastoris is the existence of a strong and tightly regulated promoter from the alcohol oxidase I (AOX1) gene. The AOX1 promoter (pAOX1) has been used to express foreign genes and to produce a variety of recombinant proteins in P. pastoris. However, some efforts have been made to develop new alternative promoters to pAOX1 to avoid the use of methanol. The glyceraldehyde-3-phosphate dehydrogenase promoter (pGAP) has been used for constitutive expression of many heterologous proteins. The pGAP-based expression system is more suitable for large-scale production because the hazard and cost associated with the storage and delivery of large volume of methanol are eliminated. Some important developments and features of this expression system will be summarized in this review.