Electrophysiology and cytology of hippocampal formation transplants in the anterior chamber of the eye. II. Cholinergic mechanisms
Electrophysiology and cytology of hippocampal formation transplants in the anterior chamber of the eye. II. Cholinergic mechanisms
复制标题
眼前房海马结构移植的电生理学和细胞学。
DOI：
10.1016/0006-8993(77)90094-4
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发表时间：
1977
期刊：
影响因子：
2.9
通讯作者：
D. Taylor
中科院分区：
文献类型：
--
作者：
B. Hoffer;A. Seiger;R. Freedman;L. Olson;D. Taylor
乙酰胆碱或3′，5′-环鸟苷单磷酸的微离子注入可有效刺激移植到眼睛前房的海马形成中的锥体神经元。对这两种药物的反应都被阿托品拮抗，并被磷酸二酯酶抑制剂IBMX和RO 20-1724增强。乙酰胆碱酯酶阳性纤维，来源于胆碱能输入虹膜，侵入移植物并在脑物质内弥漫性分支。睫状体神经节切除后，在虹膜或移植中未见此类纤维。然而，在纤毛和颈上神经节切除术后，很容易看到对乙酰胆碱和环GMP的兴奋反应。这些实验表明，正常海马的胆碱感受性和胆碱能传入纤维输入在移植中得以保留，提示环GMP可能参与了哺乳动物中枢神经系统胆碱能传递。
Microiontophoretic administration of acetylcholine or 3′,5′-cyclic guanosine monophosphate powerfully excites pyramidal neurons in hippocampal formation transplanted to the anterior chamber of the eye. Responses to both these agents are antagonized by atropine and potentiated by the phosphodiesterase inhibitors IBMX and RO 20-1724. Acetylcholinesterase-positive fibers, derived from the cholinergic input to the iris, invade the transplant and ramify diffusely within the brain substance. After ablation of the ciliary ganglion, such fibers are not seen in the iris or transplant. Excitatory responses to acetylcholine and cyclic GMP are readily seen, however, after both ciliary and superior cervical ganglionectomy. These experiments demonstrate that cholinoceptivity and cholinergic afferent fiber input, characteristic of normal hippocampus in situ, are retained in the transplant and suggest that cyclic GMP may be involved in cholinergic transmission in the mammalian central nervous system.