Archaeal and bacterial community composition of sediment and plankton from a suboxic freshwater pond
Archaeal and bacterial community composition of sediment and plankton from a suboxic freshwater pond
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DOI：
10.1016/j.resmic.2006.12.012
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发表时间：
2007-04-01
影响因子：
2.6
通讯作者：
Lopez-Garcia, Purificacion
中科院分区：
文献类型：
--
作者：
Briee, Celine;Moreira, David;Lopez-Garcia, Purificacion
以温带森林中广泛分布的一种内陆淡水系统为例，研究了一个高有机质输入的浅亚氧-缺氧淡水池塘的沉积物和浮游生物中古菌和细菌群落的组成。基于小亚基rRNA基因的分子调查显示，细菌内的谱系具有非常高的多样性，总共检测到18个门或候选部门，除了一些高度不同的未知从属关系的细菌类型。我们鉴定了变形菌门的五个亚门的成员，以及酸杆菌、疣微菌、浮菌、拟杆菌、绿菌、放线菌、厚壁菌门、绿菌、芽单胞菌、螺旋体、纤维杆菌和候选门OD 1、OP 11、TM 6、WS 1、WS 6和白蚁群1（“内细菌”）。浮游生物和沉积物的环境库中，候选司OD 1和β-变形菌分别占主导地位。古生菌也非常多样化，但只有宽古生菌的成员，包括甲烷微生物目（Methanomicrobiales）和甲烷微生物目（Methanomicrobiales），以及一些不同的谱系被确定。各种物种丰富度估计数的应用证实了浮游生物和沉积物样本的高度多样性。该池塘是一个以微生物为基础的复合生态系统，主要由外来有机物的降解维持紧密耦合的碳和硫循环。(c)2007年，Elsevier Masson SAS。All rights reserved.
We studied the composition of archaeal and bacterial communities present in the sediment and plankton of a shallow suboxic-to-anoxic freshwater pond with high organic matter input, as an example of a kind of inland freshwater system widely distributed in forests of temperate regions. Molecular surveys based on small subunit rRNA genes showed a remarkably high diversity of lineages within the Bacteria, with a total of 18 phyla or candidate divisions being detected, in addition to a few highly divergent phylotypes of unknown affiliation. We identified members of the five subdivisions of the Proteobacteria, as well as Acidobacteria, Verrucomicrobia, Planctomycetes, Bacteroidetes, Chlorobi, Actinobacteria, Firmicutes, Chloroflexi, Gemmatimonadetes, Spirochaetes, Fibrobacteres and the candidate divisions OD1, OP11, TM6, WS1, WS6 and Termite Group 1 ('' Endobacteria ''). Candidate division OD1 and beta-Proteobacteria were dominant in the environmental libraries of plankton and sediment, respectively. Archaea were also very diverse, but only members of the Euryarchaeota, including Methanosarcinales, Methanomicrobiales and some divergent lineages, were identified. The application of various species richness estimators confirmed the highly diverse nature of both plankton and sediment samples. The pond is a microbial-based complex ecosystem mainly fueled by the degradation of allochthonous organic matter that maintains tightly coupled carbon and sulfur cycles. (c) 2007 Elsevier Masson SAS. All rights reserved.