LOCALIZATION OF EXTRACELLULAR-MATRIX COMPONENTS IN DEVELOPING MOUSE SALIVARY-GLANDS BY CONFOCAL MICROSCOPY
LOCALIZATION OF EXTRACELLULAR-MATRIX COMPONENTS IN DEVELOPING MOUSE SALIVARY-GLANDS BY CONFOCAL MICROSCOPY
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DOI：
10.1002/ar.1092340315
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发表时间：
1992-11-01
影响因子：
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通讯作者：
SPOONER, BS
中科院分区：
文献类型：
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作者：
HARDMAN, P;SPOONER, BS
细胞外基质（ECM）在发育生物体中上皮-间充质相互作用中的重要性已得到充分证实。蛋白聚糖和间质胶原是上皮器官生长、形态发生和分化所必需的，这些分子的分布已被描述。然而，对发育中的上皮器官中的其他ECM大分子知之甚少。我们使用共聚焦显微镜检查层粘连蛋白，硫酸乙酰肝素（BM-1）蛋白多糖，纤连蛋白，胶原蛋白I型，IV型和V型，在小鼠胚胎唾液腺的分布。从妊娠第13天的小鼠胚胎中分离器官雏形，并培养24、48或72小时。通过间接免疫荧光染色整个封片，然后使用Zeiss激光扫描显微镜进行检查。我们发现，每个ECM成分检查有一个独特的分布，一些分子的分布随培养时间而变化。层粘连蛋白主要局限于基底膜。BM-1蛋白聚糖集中在基底膜中，并在整个间充质中形成精细网络。IV型胶原主要位于上皮的基底膜，但它也存在于整个间充质。V型胶原在24小时分布在整个间充质中，但在48小时主要位于基底膜中。I型胶原蛋白分布在整个间充质在所有的文化时间，并积累在裂缝，特别是在上皮间充质界面随着时间的增加，在文化。在整个间充质中始终观察到纤连蛋白。
The importance of the extracellular matrix (ECM) in epithelial-mesenchymal interactions in developing organisms is well established. Proteoglycans and interstitial collagens are required for the growth, morphogenesis, and differentiation of epithelial organs and the distribution of these molecules has been described. However, much less is known about other ECM macromolecules in developing epithelial organs. We used confocal microscopy to examine the distribution of laminin, heparan sulfate (BM-1) proteoglycan, fibronectin, and collagen types I, IV, and V, in mouse embryonic salivary glands. Organ rudiments were isolated from gestational day 13 mouse embryos and cultured for 24, 48, or 72 hours. Whole mounts were stained by indirect immunofluorescence and then examined using a Zeiss Laser Scan Microscope. We found that each ECM component examined had a distinct distribution and that the distribution of some molecules varied with culture time. Laminin was mainly restricted to the basement membrane. BM-1 proteoglycan was concentrated in the basement membrane and also formed a fine network throughout the mesenchyme. Type IV collagen was mainly located in the basement membrane of the epithelium, but it was also present throughout the mesenchyme. Type V collagen was distributed throughout the mesenchyme at 24 hours, but at 48 hours was principally located in the basement membrane. Type I collagen was distributed throughout the mesenchyme at all culture times, and accumulated in the clefts and particularly at the epithelial-mesenchymal interface as time in culture increased. Fibronectin was observed throughout the mesenchyme at all times.