Stem cell membrane-camouflaged bioinspired nanoparticles for targeted photodynamic therapy of lung cancer (Retracted article. See vol. 24, 2022)
Stem cell membrane-camouflaged bioinspired nanoparticles for targeted photodynamic therapy of lung cancer (Retracted article. See vol. 24, 2022)
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DOI：
10.1007/s11051-020-04915-6
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发表时间：
2020-06-19
影响因子：
2.5
通讯作者：
Wang, Luping
中科院分区：
文献类型：
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作者：
Feng, Jinjie;Wang, Shuyan;Wang, Luping
光敏剂光动力疗法（PDT）通过产生活性氧（ROS）诱导肿瘤细胞凋亡或坏死，是治疗肿瘤的有效方法。然而，光敏剂的水溶性差、血液清除快以及缺乏有效靶向仍是其令人满意的抗癌功效的严峻挑战。本研究制备了一种干细胞膜包裹的明胶纳米凝胶（Ng），它将Ng的载药能力和干细胞膜的靶向性结合起来，具有许多独特的靶向给药优势。该生物启发的药物递送系统由作为内核的疏水光敏剂二氢卟酚e6（Ce 6）-负载的明胶纳米凝胶（Ng）（Ng/Ce 6）和作为外壳的涂覆的干细胞膜囊泡（SCV）组成，记为Ng/Ce6@SCV。Ng/Ce6@SCV的平均流体动力学直径为202.7 +/-11.7nm（多分散指数（PDI）= 0.113）。Ng/Ce6@SCV能有效促进Ce 6的细胞内化，并在近红外（NIR）激光照射后在肿瘤细胞内产生足够的ROS，能有效抑制体外培养的A549肿瘤细胞的生长。Ng/Ce6@SCV给药后在肿瘤组织中表现出靶向蓄积和长期滞留，这与干细胞膜的免疫逃逸和肿瘤靶向能力有关。体内抗肿瘤活性结果还证明了NIR照射后Ng/Ce6@SCV的增强的抗肿瘤作用，其通过显著抑制原发性肿瘤生长而具有最小的副作用。所有结果表明，这种基于聚磷酸酯的生物启发纳米药物递送系统可能是一种合适的策略，用于精确和有效的癌症PDT。
Photodynamic therapy (PDT) with photosensitizers has been considered an effective strategy for treating tumors by generating reactive oxygen species (ROS) to induce tumor cells apoptosis or necrosis. However, the poor water solubility, rapid blood clearance, and lack of effective targeting of the photosensitizer are still a serious challenge for its satisfactory anti-cancer efficacy. Herein, we fabricated a stem cell membrane-camouflaged gelatin nanogels (Ng), which integrating the drug loading capacity of Ng and targeting ability of stem cell membrane, endowed with many unique advantages for targeted drug delivery. This bioinspired drug delivery system composed of hydrophobic photosensitizer, chlorin e6 (Ce6)-loaded gelatin nanogels (Ng) (Ng/Ce6), as the inner cores and coated stem cell membrane vesicles (SCV) as the outer shells, noted as Ng/Ce6@SCV. The averaged hydrodynamic diameter of Ng/Ce6@SCV was 202.7 +/- 11.7 nm (polydispersity index (PDI) = 0.113). Ng/Ce6@SCV could efficiently promote the cellular internalization of Ce6, and generate enough ROS in the tumor cells after near infrared (NIR) laser irradiation, which could efficiently suppress the growth of A549 tumor cells in vitro. After administration, Ng/Ce6@SCV exhibited targeting accumulation and long-term retention at tumor tissues, which was related to the immune escape and tumor targeting ability of the stem cell membrane. The in vivo anti-tumor activity results also demonstrated the enhanced anti-tumor effect of Ng/Ce6@SCV after NIR irradiation by significantly suppressed the primary tumor growth with minimal side effects. All the results indicated this polyphosphoester-based bioinspired nanodrug delivery system could be a suitable strategy for precise and effective PDT of cancers.