Notch signaling downstream target E(spl)mbeta is dispensable for adult midgut homeostasis in Drosophila
Notch signaling downstream target E(spl)mbeta is dispensable for adult midgut homeostasis in Drosophila
复制标题
Notch 信号下游靶标 E(spl)mbeta 对于果蝇成年中肠稳态是可有可无的。
DOI：
10.1016/j.gene.2015.01.053
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发表时间：
2015-04-10
期刊：
影响因子：
3.5
通讯作者：
Li, Zhouhua
中科院分区：
文献类型：
--
作者：
Lu, Yanfen;Li, Zhouhua
成体组织的动态平衡是由居住干细胞通过干细胞自我更新和分化的适当平衡来维持的。果蝇成体中肠含有多能肠干细胞，而Notch信号在这些肠干细胞的增殖和分化中起着关键作用。然而，Notch信号下游靶点如何调控ISC的增殖和分化仍不清楚。我们发现Notch信号下游靶基因E(SPL)、mbeta和E(SPL)Malpha在ISCs及其后代中差异表达。有趣的是，我们发现E(SPL)mbeta缺失突变体的中肠动态平衡没有受到影响。异位表达E(Spl)mbeta或E(Spl)Malpha的肠道未见明显缺陷。重要的是，我们发现在Notch突变体中观察到的ISC增殖和分化缺陷不能通过E(Spl)mbeta或E(Spl)Malpha的共同异位表达来挽救，这些数据表明ISCs的增殖和分化不是由单独的Notch下游靶点调控的，而是由不同的下游靶点共同调控的。(C)2015爱思唯尔B.V.保留所有权利。
Adult tissue homeostasis is maintained by residential stem cells through the proper balance of stem cell self-renewal and differentiation. The adult midgut of Drosophila contains multipotent intestinal stem cells (ISCs), and Notch signaling plays critical roles in the proliferation and differentiation of these ISCs. However, how Notch signaling downstream targets regulate ISC proliferation and differentiation still remains unclear. Here we find that Notch signaling downstream targets E(spl)mbeta and E(spl)malpha are differentially expressed in ISCs and their progeny. Interestingly, we find that midgut homeostasis is not affected in E(spl)mbeta null mutant. No obvious defects are observed in the intestines ectopically expressing E(spl)mbeta or E(spl)malpha. Importantly, we find that the defects in ISC proliferation and differentiation observed in Notch mutant cannot be rescued by ectopic expression of E(spl)mbeta or E(spl)malpha Together, these data indicate that the proliferation and differentiation of ISCs are not regulated by individual Notch downstream target, but by different downstream targets collectively. (C) 2015 Elsevier B.V. All rights reserved.