A novel type of RNase III family proteins in eukaryotes
A novel type of RNase III family proteins in eukaryotes
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DOI：
10.1016/s0378-1119(99)00571-5
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发表时间：
2000-03-07
期刊：
影响因子：
3.5
通讯作者：
Gill, SS
中科院分区：
文献类型：
--
作者：
Filippov, V;Solovyev, V;Gill, SS
RNase III家族是双链rna特异性内切酶，其特点是在其催化中心存在一个高度保守的9个氨基酸延伸，称为RNase III特征基序。我们分离出了drosha基因，这是黑腹果蝇家族的一个新成员。该基因的表征揭示了其序列中存在两个RNase III特征基序，这表明它能够作为单体形成活性催化中心。drosha蛋白还含有一个功能未知的825个氨基酸的n端；对已知的drosha蛋白同源物的搜索显示，它与秀丽隐杆线虫基因组测序中鉴定的两个相邻的注释基因相似。通过Fgenesh程序对这些基因的基因组区域进行分析，并对其EST cDNA克隆进行测序，结果表明该区域只编码一个基因。这一新发现的线虫基因组基因在整个蛋白序列上与果蝇具有高度的相似性。在已沉积的人类cDNA序列中也发现了潜在的drosha同源物。将这些drosha蛋白与RNase III家族的其他成员进行比较表明，它们在该家族中形成了一个新的蛋白质组。(C) 2000由Elsevier Science B.V.出版，版权所有。
The RNase III family of double-stranded RNA-specific endonucleases is characterized by the presence of a highly conserved 9 amino acid stretch in their catalytic center known as the RNase III signature motif. We isolated the drosha gene, a new member of this family in Drosophila melanogaster. Characterization of this gene revealed the presence of two RNase III signature motifs in its sequence that map indicate that it is capable of forming an active catalytic center as a monomer. The drosha protein also contains an 825 amino acid N-terminus with an unknown function; A search for the known homologues of the drosha protein revealed that it has a similarity to two adjacent annotated genes identified during C. elegans genome sequencing. Analysis of the genomic region of these genes by the Fgenesh program and sequencing of the EST cDNA clone derived from it revealed that this region encodes only one gene. This newly identified gene in nematode genome shares a high similarity to Drosophila drosha throughout its entire protein sequence. A potential drosha homologue is also found among the deposited human cDNA sequences. A comparison of these drosha proteins to other members of the RNase III family indicates that they form a new group of proteins within this family. (C) 2000 Published by Elsevier Science B.V. All rights reserved.