HIGH FIDELITY DNA-SYNTHESIS BY THE THERMUS-AQUATICUS DNA-POLYMERASE
HIGH FIDELITY DNA-SYNTHESIS BY THE THERMUS-AQUATICUS DNA-POLYMERASE
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DOI：
10.1093/nar/18.13.3739
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发表时间：
1990-07-11
影响因子：
14.9
通讯作者：
KUNKEL, TA
中科院分区：
文献类型：
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作者：
ECKERT, KA;KUNKEL, TA
我们证明，尽管缺少一个3”。5“校对核酸外切酶，热水（Taq） DNA聚合酶可以在体外催化高度精确的DNA合成。在规定的反应条件下，每个核苷酸在70度下聚合的错误率。对于碱基替换错误，C可低至10-5，对于移码错误，C可低至10-6。在lac z . α的单轮DNA合成过程中产生的突变频率。Taq聚合酶对dNTP浓度、pH和MgCl2相对于反应中三磷酸脱氧核苷酸总浓度的变化有响应。的碱基替换和移码错误率
We demonstrate that despite lacking a 3''.fwdarw.5'' proofreading exonuclease, the Thermus aquaticus (Taq) DNA polymerase can catalyze highly accurate DNA synthesis in vitro. Under defined reaction conditions, the error rate per nucleotide polymerized at 70.degree. C can be as low as 10-5 for base substitution errors and 10-6 for frameshift errors. The frequency of mutations produced during a single round of DNA synthesis of the lac Z.alpha. gene by Taq polymerase responds to changes in dNTP concentration, pH, and the concentration of MgCl2 relative to the total concentration of deoxynucleotide triphosphates present in the reaction. Both base substitution and frameshift error rates of