Microfluidic Chip-Based Online Electrochemical Detecting System for Continuous and Simultaneous Monitoring of Ascorbate and Mg2+ in Rat Brain
Microfluidic Chip-Based Online Electrochemical Detecting System for Continuous and Simultaneous Monitoring of Ascorbate and Mg2+ in Rat Brain
复制标题
基于微流控芯片的连续同步监测大鼠脑内抗坏血酸和镁的在线电化学检测系统
DOI：
10.1021/ac401727d
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发表时间：
2013-08-06
影响因子：
7.4
通讯作者：
Mao, Lanqun
中科院分区：
文献类型：
--
作者：
Gao, Xia;Yu, Ping;Mao, Lanqun
本研究展示了一种基于微流控芯片的在线电化学检测系统，用于大鼠大脑中抗坏血酸和Mg2+的体内连续和同时监测。在该系统中，微流控芯片被用作两种物种的检测器。为制作该检测器，将单通道微流控芯片以氧化铟锡（ITO）电极为工作电极，Ag/AgCl导线为参比电极，不锈钢管为反电极，制成电化学流动电池。通过滴涂单壁碳纳米管（SWNTs）和在ITO电极上聚合甲苯胺蓝O （polyTBO）膜，分别实现了抗坏血酸和Mg2+的选择性检测。此外，还精心设计了swnt修饰电极和polytbo修饰电极的排列和溶液引入模式，以避免两个电极之间的串扰。以基于微流控芯片的电化学流动电池为检测器，将基于微流控芯片的电化学流动电池与体内微透析直接集成，成功建立了在线电化学检测系统。微流控系统表现出抗坏血酸5 ~ 100 μ M和Me 100 ~ 2000 μ M的线性关系。此外，该系统在连续流系统中同时测量抗坏血酸和Mg2+具有高选择性、稳定性和良好的重现性。这些优良的特性使该系统在大鼠脑内抗坏血酸和Mg2+的连续和同时在线监测方面具有很大的潜力。
This study demonstrates a microfluidic chip-based online electrochemical detecting system for in vivo continuous and simultaneous monitoring of ascorbate and Mg2+ in rat brain. In this system, a microfluidic chip is used as the detector for both species. To fabricate the detector, a single-channel microfluidic chip is developed into an electrochemical flow cell by incorporating the chip with an indium-tin oxide (ITO) electrode as working electrode, an Ag/AgCl wire as reference electrode, and a stainless steel tube as counter electrode. Selective detection of ascorbate and Mg2+ is achieved by drop-coating single-walled carbon nanotubes (SWNTs) and polymerizing toluidine blue O (polyTBO) film onto the ITO electrode, respectively. Moreover, the alignment of SWNT-modified and polyTBO-modified electrodes and the solution introduction pattern are carefully designed to avoid any cross talk between two electrodes. With the microfluidic chip-based electrochemical flow cell as the detector, an online electrochemical detecting system is successfully established by directly integrating the microfluidic chip-based electrochemical flow cell with in vivo microdialysis. The microfluidic system exhibits sensing properties with a linear relationship from 5 to 100 mu M for ascorbate and from 100 to 2000 mu M for Me. Moreover, this system demonstrates a high selectivity and stability and good reproducibility for simultaneous measurements of ascorbate and Mg2+ in a continuous-flow system. These excellent properties substantially render this system great potential for continuous and simultaneous online monitoring of ascorbate and Mg2+ in rat brain.