Development of a novel dual-functional lateral-flow sensor for on-site detection of small molecule analytes
Development of a novel dual-functional lateral-flow sensor for on-site detection of small molecule analytes
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DOI：
10.1016/j.snb.2014.06.043
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发表时间：
2014-11-01
影响因子：
8.4
通讯作者：
Tang, Yong
中科院分区：
文献类型：
--
作者：
Fu, Qiangqiang;Liang, Jiajie;Tang, Yong
最近，需要更灵敏的方法来分析小分子分析物。侧向流传感器（LFS）已被用作可靠，快速和成本效益的现场筛选技术来检测小分子分析物。然而，用于小分子分析物的可用LFS是竞争性的，并且在“关闭”模式下进行，允许通过肉眼进行低灵敏度检测。在这项研究中，我们使用双功能LFS监测小分子分析物（克伦特罗CL），这是基于金纳米粒子（AuNPs）的荧光猝灭效应。该传感器最重要的特点是它的双重功能，即在激发光下“打开”LFS和在自然光下关闭LFS。在激发光下，“开启”LFS的灵敏度达到0.04 ng/mL;然而，在自然光下，它们表现为具有5.0 ng/mL灵敏度的普通“关闭”AuNPs LFS。这种新型的LFS可用于传统或高灵敏度的工具，以检测各种小的分析物。(C)2014爱思唯尔有限公司版权所有。
Recently, more sensitive methods for analyzing small molecule analytes were required. Lateral-flow sensors (LFSs) have been used as reliable, rapid and cost-effective on-site screening technique to detect small molecule analytes. However, available LFSs for small molecule analytes were competitive and performed in "turn-off" mode, allowing low-sensitivity detection by the naked eye. In this study, we used dual-functional LFSs for monitoring small molecule analytes (clenbuterol CL), which based a fluorescence-quenching effect of gold nanoparticles (AuNPs). The most important feature of this sensor is its dual function as "turn-on" LFSs under excitation light and turn-off LFSs under natural light. The sensitivity of "turn-on" LFSs reached 0.04 ng/m under excitation light; however, under natural light they performed as common "turn-off" AuNPs LFSs with 5.0 ng/mL sensitivity. This novel LFS can be used in either traditional or highly sensitive tools to detect a variety of small analytes. (C) 2014 Elsevier B.V. All rights reserved.