Neuropeptide Y and N-methyl-D-aspartic acid interact within the suprachiasiviatic nuclei to alter circadian phase
Neuropeptide Y and N-methyl-D-aspartic acid interact within the suprachiasiviatic nuclei to alter circadian phase
复制标题
DOI：
10.1016/j.neuroscience.2004.04.018
复制
发表时间：
2004-01-01
期刊：
影响因子：
3.3
通讯作者：
Albers, HE
中科院分区：
文献类型：
--
作者：
Gamble, KL;Novak, CM;Albers, HE
昼夜节律通过暴露于光刺激和非光刺激而重置。谷氨酸似乎是向位于视交叉上核的生物钟传递光刺激的主要神经递质。也有大量证据表明神经肽Y （NPY）介导至少一些非光刺激对生物钟的影响。本研究的目的是研究NPY和谷氨酸受体激活如何相互作用以重置生物钟的阶段。在主观日期间微量注射谷氨酸激动剂n -甲基- d -天冬氨酸（NMDA）可显著降低npy诱导的期进展。在主观深夜，NMDA诱导的光样相提前，微注射NPY后明显减少。微注射NPY可以抑制nmda诱导的主观深夜的相推进，即使是在钠依赖的动作电位被河鲀毒素抑制的情况下。这些数据支持了一个假设，即在主观的夜晚，NPY和NMDA作用于相同的时钟细胞，或者通过不需要动作电位的机制作用于与时钟细胞通信的细胞。尽管NPY和NMDA在白天和晚上似乎都相互抑制，但这种抑制的机制在白天和晚上似乎是不同的。(c) 2004 ibro。Elsevier Ltd.出版。版权所有。
Circadian rhythms are reset by exposure to photic stimuli and nonphotic stimuli. Glutamate appears to be the primary neurotransmitter that communicates photic stimuli to the circadian clock located in the suprachiasmatic nucleus. There is also substantial evidence that neuropeptide Y (NPY) mediates the effects of at least some nonphotic stimuli on the circadian clock. The purpose of this study was to investigate how NPY and glutamate receptor activation interact to reset the phase of the circadian clock. Microinjection of the glutamate agonist N-methyl-D-aspartic acid (NMDA) during the subjective day significantly decreased NPY-induced phase advances. During the late subjective night, NMDA induced light-like phase advances, which were significantly reduced by microinjection of NPY. Microinjection of NPY inhibited NMDA-induced phase advances during the late subjective night, even when sodium-dependent action potentials were inhibited by tetrodotoxin. These data support the hypothesis that, during the subjective night, NPY and NMDA act on the same clock cells or on cells that communicate with clock cells by mechanisms not requiring action potentials. Although NPY and NMDA appear to be mutually inhibitory during both the day and the night, the mechanisms of this inhibition appear to be different during the day versus the night. (C) 2004 IBRO. Published by Elsevier Ltd. All rights reserved.