Identification of protein network alterations upon retinal ischemia-reperfusion injury by quantitative proteomics using a Rattus norvegicus model.
Identification of protein network alterations upon retinal ischemia-reperfusion injury by quantitative proteomics using a Rattus norvegicus model.
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DOI：
10.1371/journal.pone.0116453
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Guo L
中科院分区：
文献类型：
--
作者：
Tian H;Wang L;Cai R;Zheng L;Guo L
视网膜缺血是与包括糖尿病视网膜病变在内的几种眼部疾病相关的常见特征。在这项研究中，我们研究了视网膜缺血和再灌注（I/R）损伤的蛋白质水平的影响，通过定量鸟枪策略，使用稳定的同位素二甲基标记结合LC-MS/MS分析。基于1088个蛋白质的相对定量数据，234个蛋白质在I/R损伤后显示出大于1.5倍的变化，其中194个蛋白质表达上调，40个蛋白质表达下调。基因本体分析显示，I/R损伤后，与代谢过程相关的蛋白表达增加，而与细胞通讯、系统过程和转运相关的蛋白表达减少。在上调的蛋白质中鉴定了由许多蛋白酶抑制剂组成的核糖体蛋白质网络和分泌蛋白质网络，尽管I/R损伤后哺乳动物雷帕霉素靶蛋白（mTOR）通路受到抑制。发现突触相关蛋白网络显著下调，暗示视网膜I/R损伤后神经元功能降低。本研究结果为视网膜缺血的研究提供了新的系统生物学线索。
Retinal ischemia is a common feature associated with several ocular diseases, including diabetic retinopathy. In this study, we investigated the effect of a retinal ischemia and reperfusion (I/R) injury on protein levels via a quantitative shotgun strategy using stable isotope dimethyl labeling combined with LC-MS/MS analysis. Based on the relative quantitation data of 1088 proteins, 234 proteins showed a greater than 1.5-fold change following I/R injury, 194 of which were up-regulated and 40 were down-regulated. Gene ontology analysis revealed that after I/R injury, there was an increase in the metabolic-process related proteins but a decline in cell communication, system process and transport-related proteins. A ribosome protein network and a secreted protein network consisting of many protease inhibitors were identified among the up-regulated proteins, despite a suppression of the mammalian target of rapamycin (mTOR) pathway following the I/R injury. A synaptic-related protein network was found to be significantly down-regulated, implicating a functional reduction of neurons following a retinal I/R injury. Our results provide new systems-biology clues for the study of retinal ischemia.
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影响因子：
3.7
作者：
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通讯作者：
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DOI：
10.1016/j.jchromb.2010.11.004
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