Seasonal changes in the Plasmodium falciparum population in individuals and their relationship to clinical malaria:: a longitudinal study in a Sudanese village
Seasonal changes in the Plasmodium falciparum population in individuals and their relationship to clinical malaria:: a longitudinal study in a Sudanese village
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DOI：
10.1017/s0031182098002650
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发表时间：
1998-06-01
期刊：
影响因子：
2.4
通讯作者：
Arnot, DE
中科院分区：
文献类型：
--
作者：
Roper, C;Richardson, W;Arnot, DE
在1993-1996年的三个疟疾季节，对苏丹Daraweesh村的居民进行了恶性疟原虫感染和疟疾发病率监测。在一系列横断面调查中，通过显微镜和聚合酶链式反应(PCR)检测疟疾寄生虫。聚合酶链式反应揭示了旱季的亚显微感染，特别是在成功进行药物治疗后从疟疾发作中恢复的个人中。通过分析MSP-1和GLUP两个基因座的等位基因多态，以及参考这些数据检验的两个假设，对比了临床和亚临床感染：临床疟疾与感染以前在该宿主中未检测到的新寄生虫基因型有关，或者，临床疟疾发作与感染中克隆数量的增加有关。我们在临床分离株中检测到更多的混合感染，但没有发现在旱季携带寄生虫的人在接下来的疟疾季节患病风险增加。疾病与新的寄生虫基因类型的出现有明显的联系。
Residents of Daraweesh village in Sudan were monitored for Plasmodium falciparum infection and malaria morbidity in 3 malaria seasons from 1993 to 1996. Malaria parasites were detected microscopically and by polymerase chain reaction (PCR) in a series of cross-sectional surveys. PCR revealed submicroscopical infections during the dry season, particularly among individuals who had recovered from a malaria episode following successful drug treatment. Clinical and subclinical infections were contrasted by assaying for allelic polymorphism at 2 gene loci, MSP-1 and GLURP and 2 hypotheses examined with reference to these data: that clinical malaria is associated with infection with novel parasite genotypes not previously detected in that host, or alternatively, that clinical malaria episodes are associated with an increased number of clones in an infection. We detected more mixed infections among clinical isolates, but people carrying parasites during the dry season were not found to have an increased risk of disease in the following malaria season. There was a clear association of disease with the appearance of novel parasite genotypes.