Bacterial microbiota associated with flower pollen is influenced by pollination type, and shows a high degree of diversity and species-specificity
Bacterial microbiota associated with flower pollen is influenced by pollination type, and shows a high degree of diversity and species-specificity
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DOI：
10.1111/1462-2920.13524
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发表时间：
2016-12-01
影响因子：
5.1
通讯作者：
Schnell, Sylvia
中科院分区：
文献类型：
--
作者：
Manirajan, Binoy Ambika;Ratering, Stefan;Schnell, Sylvia
不同的微生物在不同的植物微生境中定居，如根际和叶圈，并对宿主起着关键作用。然而，与花粉相关的细菌研究很少，尽管它具有生态、商业和医学上的相关性。由于花粉的结构和营养成分，它提供了一个独特的微生境。通过培养、高通量测序和显微镜观察等方法，研究了桦树、黑麦、油菜和秋番红花花粉的细菌丰度、群落结构、多样性和定殖格局。培养出的细菌属于变形杆菌、放线杆菌和粘菌，在种水平上不同花粉种之间存在显著差异。16S rRNA基因扩增子文库的高通量测序表明，变形杆菌是所有花粉物种中的优势门，其次是放线杆菌、酸性细菌和绒毛杆菌。植物种类和授粉类型对花粉微生物区系的结构和多样性均有显著影响。与风媒传粉物种相比，昆虫传粉物种具有更相似的微生物区系，这表明昆虫媒介的平坦效应。扫描电子显微镜以及荧光原位杂交结合共聚焦激光扫描显微镜(FISH-CLSM)表明，顶盖表面是细菌定植的首选生态位。这项工作是对花粉微生物学最全面的研究，并有力地增加了我们对一种较少被研究的植物微生境的了解。
Diverse microorganisms colonise the different plant-microhabitats, such as rhizosphere and phyllosphere, and play key roles for the host. However, bacteria associated with pollen are poorly investigated, despite its ecological, commercial and medical relevance. Due to structure and nutritive composition, pollen provides a unique microhabitat. Here the bacterial abundance, community structure, diversity and colonization pattern of birch, rye, rapes and autumn crocus pollens were examined, by using cultivation, high-throughput sequencing and microscopy. Cultivated bacteria belonged to Proteobacteria, Actinobacteria and Firmicutes, with remarkable differences at species level between pollen species. High-throughput sequencing of 16S rRNA gene amplicon libraries showed Proteobacteria as the dominant phylum in all pollen species, followed by Actinobacteria, Acidobacteria and Firmicutes. Both plant species and pollination type significant influenced structure and diversity of the pollen microbiota. The insect-pollinated species possessed a more similar microbiota in comparison to the wind-pollinated ones, suggesting a levelling effect by insect vectors. Scanning electron microscopy as well as fluorescent in situ hybridisation coupled with confocal laser scanning microscopy (FISH-CLSM) indicated the tectum surface as the preferred niche of bacterial colonisation. This work is the most comprehensive study of pollen microbiology, and strongly increases our knowledge on one of the less investigated plant-microhabitats.