Streptococcus mutans and Streptococcus sobrinus colonization and caries experience in 3- and 5-year-old Thai children.
Streptococcus mutans and Streptococcus sobrinus colonization and caries experience in 3- and 5-year-old Thai children.
复制标题
在3岁和5岁的泰国儿童中，链球菌和Sobrinus链球菌定植和龋齿经历。
DOI：
10.1007/s00784-015-1437-0
复制
发表时间：
2015-11
影响因子：
3.4
通讯作者：
Li Y
中科院分区：
文献类型：
--
作者：
Saraithong P;Pattanaporn K;Chen Z;Khongkhunthian S;Laohapensang P;Chhun N;Pattanaporn W;Gaw HY;Li Y
本研究的目的是检查龈上菌斑样本中变形链球菌和远缘链球菌的定植，并确定其与泰国儿童早期龋齿（ECC）患病率的相关性。共有344名3岁和5岁的泰国儿童被邀请参加这项研究。检查儿童的龋病状况。收集龈上菌斑样本。采用实时荧光定量PCR技术检测S.和S. sobrinus。85%的儿童被S.变形链球菌占50.9%。sobrinus。3岁和5岁儿童ECC的患病率分别为43.8%和56.2%，且与S。和S. sobrinus。ECC的严重程度与两种细菌的DNA水平增加显著相关。S.和S.远缘型（Sm+/Sb+）儿童发生ECC的可能性是Sm−/Sb+或Sm−/Sb−儿童的8倍或44倍。研究证据进一步表明，儿童同时被S。和S. sobrinus有更高的风险为ECC。基于分子的qPCR可用于检测和定量S。和S.用于ECC风险评估的流行病学和临床研究的sobrinus定植。
The aim of this study was to examine the colonization of Streptococcus mutans and Streptococcus sobrinus in supra-gingival plaque samples and to determine their correlation with the prevalence of early childhood caries (ECC) in Thai children. A total of 344 Thai children, ages 3 and 5 years, were invited to participate in this study. Caries status of the children was examined. Supra-gingival plaque samples were collected. Quantitative real-time PCR was performed to evaluate DNA levels of S. mutans and S. sobrinus. Eighty-five percent of the children were colonized by S. mutans and 50.9 % of them were colonized by S. sobrinus. The prevalence of ECC was 43.8 % and 56.2 % among 3- and 5-year-old children, respectively, and was significantly associated with the presence of S. mutans and S. sobrinus. The severity of ECC was significantly correlated with increased DNA levels of the two bacteria. Children who were positive for S. mutans and S. sobrinus (Sm+/Sb+) were 8 times or 44 times more likely to experience ECC than children who were Sm−/Sb+or were Sm−/Sb−. The study evidence further suggest that children colonized by both S. mutans and S. sobrinus are at the higher risk for ECC. Molecular-based qPCR can be used to detect and quantify S. mutans and S. sobrinus colonization for epidemiological and clinical studies for ECC risk assessment.