The surface modification of silver nanoparticles by phosphoryl disulfides for improved biocompatibility and intracellular uptake
The surface modification of silver nanoparticles by phosphoryl disulfides for improved biocompatibility and intracellular uptake
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DOI：
10.1016/j.biomaterials.2007.12.032
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发表时间：
2008-04-01
期刊：
影响因子：
14
通讯作者：
Chen, Ching-Jung
中科院分区：
文献类型：
--
作者：
Chung, Yi-Chang;Chen, I-Han;Chen, Ching-Jung
为了提高金属纳米粒子的生物相容性和细胞亲和性，使其应用于生物传感和药物释放领域，我们制备了含二硫键的磷脂衍生物对银纳米粒子进行表面修饰。通过加入硼氢化钠同时还原衍生物的二硫键和银离子，生成的巯基可以立即与新生的银原子反应生成纳米团簇。组装体由磷酸胆碱（PC）或磷酸乙醇胺（PE）头基组成，这使得银簇具有生物相容性。透射电子显微镜（TEM）和光学吸收光谱辅助调制反应条件，表明表面活性剂/银的比例为0.4导致形成均匀，分散良好的球形颗粒直径约3.8 nm。X射线光电子能谱和红外光谱也说明了纳米粒子的元素和分子结构。若丹明染料插入到表面活性剂层中使得纳米颗粒能够用作荧光探针。在细胞培养试验中，纳米颗粒在短时间的孵育中内化到血小板或成纤维细胞中，而不损害细胞。(C)2008爱思唯尔有限公司版权所有。
In order to enhance the biocompatibility and cell affinity of metal nanoparticles for biosensing and drug delivering applications, we prepared the phospholipid derivatives containing disulfide groups to modify silver nanoparticle surfaces. By adding sodium borohydride to reduce both disulfide bonds of the derivatives and silver ions simultaneously, the generated thiol groups can be reacted with newborn silver atoms immediately to generate nanoclusters. The assemblies consisted of either phosphorylcholine (PC) or phosphorylethanolamine (PE) head groups, which made the silver clusters biocompatibile. Transmission electron microscope (TEM) and optical absorption spectra assisted in modulating reaction conditions, demonstrating that a surfactant/Ag ratio of 0.4 led to the formation of uniform, well-dispersed spherical particles about 3.8 nm in diameter. X-ray photoelectron spectra and infrared spectra also illustrated the elemental and molecular structures of nanoparticles. The insertion of rhodamine dye into the surfactant layer enabled the nanoparticles to be used as a fluorescent probe. In cell culture tests, the nanoparticles were internalized into platelet or fibroblast cells in a short period of incubation without harming the cells. (C) 2008 Elsevier Ltd. All rights reserved.