Recent trends in nanopores for biotechnology.
Recent trends in nanopores for biotechnology.
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DOI：
10.1016/j.copbio.2012.11.008
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发表时间：
2013-08
影响因子：
7.7
通讯作者：
Wanunu M
中科院分区：
文献类型：
--
作者：
Stoloff DH;Wanunu M
纳米孔技术采用绝缘膜中的纳米级孔来对溶液中的高通量单个生物分子进行随机感测。纳米孔检测原理的普遍性和单分子检测的容易性表明纳米孔在生物技术中的许多潜在应用。最近的进展已经与纳米孔的制造和复杂性，以及与DNA/蛋白质映射，生物分子结构分析，蛋白质检测和DNA测序的应用。此外，已经提出了DNA测序装置的概念，并进行了计算工作。纳米孔领域的状态正在成熟，如果有合适的纳米孔类型和操作条件，几乎每一种应用都可以在速度、成本和质量方面彻底改变医学。本文综述了近2-3年来纳米孔在生物技术中的应用进展。
Nanopore technology employs a nanoscale hole in an insulating membrane to stochastically sense with high throughput individual biomolecules in solution. The generality of the nanopore detection principle and the ease of single-molecule detection suggest many potential applications of nanopores in biotechnology. Recent progress has been made with nanopore fabrication and sophistication, as well as with applications in DNA/protein mapping, biomolecular structure analysis, protein detection, and DNA sequencing. In addition, concepts for DNA sequencing devices have been suggested, and computational efforts have been made. The state of the nanopore field is maturing and given the right type of nanopore and operating conditions, nearly every application could revolutionize medicine in terms of speed, cost, and quality. In this review, we summarize progress in nanopores for biotechnological applications over the past 2–3 years.