Molecular adaptation of sourdough Lactobacillus plantarum DC400 under co-cultivation with other lactobacilli
Molecular adaptation of sourdough Lactobacillus plantarum DC400 under co-cultivation with other lactobacilli
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DOI：
10.1016/j.resmic.2009.04.006
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发表时间：
2009-06-01
影响因子：
2.6
通讯作者：
Gobbetti, Marco
中科院分区：
文献类型：
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作者：
Di Cagno, Raffaella;De Angelis, Maria;Gobbetti, Marco
本工作旨在研究植物乳杆菌DC400与其他酸面菌共培养时群体感应(QS)的分子机制。与桑氏乳杆菌DPPMA174和罗氏乳杆菌AT共培养时，对植物乳杆菌DC400的生长和存活没有影响，但双向凝胶电泳法分析表明，共培养条件下植物乳杆菌DC400的蛋白质表达水平增加。虽然在两种共培养中都有几种蛋白被诱导，但在与玫瑰乳杆菌AT过度表达的蛋白的共同培养中，与QS和胁迫反应机制相关的蛋白被发现是最高的：DNAK，GroEL，30S核糖体蛋白S1和S6，ATP合成酶亚基β，腺苷蛋氨酸合成酶(MetK)，磷酸丙酮酸水合酶，磷酸甘油酸激酶，延伸因子Tu，可能依赖于锰的无机焦磷酸酶，D-乳酸脱氢酶，三糖磷酸异构酶，果糖-二磷酸缩醛酶和核苷-二磷酸激酶。实时荧光定量聚合酶链式反应显示，在共培养过程中，植物乳杆菌DC400的LuxS基因的表达也受到了影响。根据共培养过程中MetK和LuxS的过度表达，AI-2类物质的合成也受到微生物共培养类型的影响。本研究表明，植物乳杆菌中一些与QS相关的基因/蛋白的表达受到其他酸面团乳酸菌共培养的影响。(C)2009年爱思唯尔·马森公司。版权所有。
This work was aimed at investigating the molecular mechanisms of Quorum Sensing (QS) in Lactobacillus plantarum DC400 when co-cultured with other sourdough lactobacilli. The growth and survival of L. plantarum DC400 was not affected when co-cultivated with Lactobacillus sanfranciscensis DPPMA174 or Lactobacillus rossiae AT Nevertheless, 2-DE analysis showed that the level of protein expression of L. plantarum DC400 increased under co-culture conditions. Although several proteins were commonly induced in both co-cultures, the highest induction was found in co-culture with L. rossiae AT Overexpressed proteins, related to QS and stress response mechanisms, were identified: DnaK, GroEL, 30S ribosomal protein S1 and S6, ATP synthase subunit beta, adenosylmethionine synthetase (MetK), phosphopyruvate hydratase, phosphoglycerate kinase, elongation factor Tu, putative manganese-dependent inorganic pyrophosphatase, D-lactate dehydrogenase, triosephosphate isomerase, fructose-bisphosphate aldolase and nucleoside-diphosphate kinase. As shown by real-time PCR, expression of the luxS gene of L. plantarum DC400 was also affected during co-cultivation. According to overexpression of MetK and luxS during co-cultivation, synthesis of AI-2-like substances was also influenced by the type of microbial co-cultures.This study showed that expression of some genes/proteins, also QS-related, in L. plantarum was influenced by co-cultivation of other sourdough lactobacilli. (C) 2009 Elsevier Masson SAS. All rights reserved.