Analyses of microglia effector function using CX3CR1-GFP knock-in mice.
Analyses of microglia effector function using CX3CR1-GFP knock-in mice.
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DOI：
10.1007/978-1-62703-520-0_27
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发表时间：
2013
期刊：
影响因子：
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通讯作者：
Cardona, Astrid E
中科院分区：
文献类型：
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作者：
Garcia, Jenny A;Cardona, Sandra M;Cardona, Astrid E
骨髓辐射嵌合小鼠的产生是研究中枢神经系统（CNS）炎症时使用的有益工具。人们普遍认为，血液来源的祖细胞能够在慢性疾病和严重损伤期间填充CNS，然而，它们在这样做时既不一致也不有效。缺乏适当的招募可以解释慢性神经退行性疾病中毒性蛋白增加后恢复和修复的延迟。随着骨髓嵌合小鼠的巧妙开发，这些问题中的一些可以得到解决，并允许我们假设可能导致医学应用的进一步影响和可能的机制。骨髓嵌合小鼠通常用于区分特定突变的内在效应与外在效应。在我们的案例中，嵌合体帮助我们更好地理解CX 3CR 1在小胶质细胞和外周髓样细胞中的作用。为了检测细胞对骨髓细胞分化的自主作用，CX 3CR 1缺陷型小鼠用作供体，野生型小鼠用作受体。为了检测对“免疫细胞环境”的影响，野生型供体被用于转移到Cx 3cr 1 −/−受体中。由此产生的嵌合小鼠通常用于分析小胶质细胞的运动性，神经炎症的调节和持久性。这项技术可以应用于广泛的研究，从神经退行性疾病到病毒和寄生虫的致病性以及介于两者之间的一切。该方案描述了产生嵌合小鼠并分析CX 3CR 1在骨髓放射嵌合体中CNS炎症中的作用的方法。
The generation of bone marrow radiation chimeric mice is a beneficial tool to utilize when studying inflammation of the central nervous system (CNS). It is widely accepted that blood-derived progenitors are capable of populating the CNS during chronic diseases and severe injuries however, they are neither consistent nor efficient in doing so. The lack of the appropriate recruitment could explain delays in recovery and repair after an increase of toxic proteins in chronic neurodegenerative diseases. With the ingenious development of bone marrow chimeric mice, some of these concerns can be addressed and allow us to hypothesize about further implications and possible mechanisms that may lead to medicinal applications. Bone marrow chimeric mice are often used to distinguish the intrinsic versus extrinsic effects of specific mutations. In our case, chimeras help us to better understand the role of CX3CR1 in microglia and peripheral myeloid cells. To detect cell autonomous effects on myeloid cell differentiation, CX3CR1-deficient mice are used as donors and wild type mice are used as recipients. In order to detect effects on the “immune cell environment,” wild type donors are used for the transfer into Cx3cr1−/− recipients. The resulting chimeric mice are often used for the analysis of microglial motility, regulation of neuroinflammation and persistence. This technique can be applied to a broad spectrum of research ranging from neurodegenerative diseases to viral and parasitic pathogenicity and everything in between. This protocol describes the approach to generate chimeric mice and analyze the role of CX3CR1 in CNS inflammation in bone marrow radiation chimeras.