Physical exercise intensity can be related to plasma glutathione levels
Physical exercise intensity can be related to plasma glutathione levels
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DOI：
10.1007/bf03179808
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发表时间：
2001-03-01
影响因子：
3.4
通讯作者：
Rufini, S
中科院分区：
文献类型：
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作者：
Gambelunghe, C;Rossi, R;Rufini, S
本研究旨在探讨不同运动方式对血浆谷胱甘肽水平的影响。雄性Wistar大鼠随机分为4组：步行组（W组，n=6），以0.8m/min的速度步行45 min;慢速跑组（SR组，n=6），以4 m/min的速度跑45 min;快速跑组（FR组，n= 6），以8 m/min的速度跑60 min;所有动物在运动后处死，用高效液相色谱（HPLC）荧光检测器测定血浆样品中还原型谷胱甘肽（GSH）的水平。与对照组相比，运动并没有改变W组的GSH血浆水平。在SR组和FR组的血浆样品中观察到降低血液GSH的趋势，体育锻炼导致GSH血浆水平急剧下降。这些数据表明，在轻度体育锻炼期间，活性氧（ROS）的产生量较低，对抗氧化防御（如GSH的氧化）的要求较低。在FR大鼠中观察到的GSH水平急剧下降表明存在能够改变血液抗氧化剂特征的氧化应激。我们的研究结果表明，谷胱甘肽在血液中起着重要的抗氧化作用，在激烈的体育锻炼，其修改是密切相关的运动强度。
The aim of the present study was to examine the effect of different kinds of physical exercise on plasma glutathione levels. Male Wistar rats were randomly divided into four groups: In walking group (W; n=6), rats were trained to walk 0.8 m/min for 45 min; slow running group (SR; n=6) were trained to run 4 m/min for 45 min; fast running group (FR; n=6) ran 8m/min for 60 min and control rats (C; n=6) remained in their home cages. All animals were sacrificed after exercise and the levels of reduced glutathione (GSH) in plasma samples determined by high performance liquid chromatography (HPLC) with a fluorescent detector. Compared to controls, exercise did not change GSH plasma levels of the W group. A tendency to decrease blood GSH was observed in plasma samples of the SR group and in the FR group, physical exercise resulted in a dramatic decrease in GSH plasma levels. These data suggest that during light physical exercise there is a low production of reactive oxygen species (ROS) with a low request for antioxidant defence such as oxidation of GSH. The dramatic decrease observed in GSH levels in FR rats would indicate the presence of oxidative stress able to modify blood antioxidant profiles. Our results suggest that GSH plays a central antioxidant role in blood during intensive physical exercise and that its modifications are closely related to exercise intensity.