Effects of DNA Methylation on Growth and Development of Rehmannia glutinosa
Effects of DNA Methylation on Growth and Development of Rehmannia glutinosa
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DNA甲基化对地黄生长发育的影响
DOI：
10.17957/ijab/15.0745
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发表时间：
2018-01-01
影响因子：
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通讯作者：
Zhou, Yanqing
中科院分区：
文献类型：
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作者：
Duan, Hongying;Li, Jiaojiao;Zhou, Yanqing
在本研究中，5 mC含量和甲基化水平均随着紫茎泽兰的生长而沿着增加，但叶和根中DNA甲基化程度不同，叶和根中的半甲基化水平均高于全甲基化水平，其中根中的甲基化水平极显著。此外，5-azaC还能抑制R.与对照相比，5-azaC含量虽有所减少，且随着5-azaC浓度的增加呈下降趋势，但仍随R.在第四阶段达到顶峰。此外，5-azaC处理后，R.拟南芥具有明显的多态性，甲基化状态会发生变化，出现明显的去甲基化，DNA去甲基化程度随着5-azaC浓度的增加而沿着增加。进一步分析发现，在5-azaC处理下，株高和叶长均低于对照，但仍沿着生长而增加。而R.随着5-azaC浓度的增加，紫穗槐的生长没有出现明显的沿着的变化。因此，在DNA甲基化降低的情况下，R.紫茎泽兰表现出明显的矮化现象，叶片变小，说明适度的去甲基化会抑制紫茎泽兰的生长发育。这可能与基因组DNA甲基化状态密切相关。(c)2018 Friends Science出版社
In this study, 5mC content and methylation level increased along with the growth of Rehmannia glutinosa, but degree of DNA methylation was different in leaf and root, and hemi-methylation level was both higher than full-methylation level in leaf or root, which was very significant in root. Furthermore, 5-azaC could inhibit DNA methylation of R. glutinosa, compared with the control, although 5mC content was less and showed a decreasing trend along with the increase of 5-azaC concentration, it still increased along with the growth of R. glutinosa and reached the peak in Stage IV. In addition, as treated with 5-azaC, DNA methylation of R. glutinosa had significant polymorphism, methylation status would change and appear significant demethylation, and degree of DNA demethylation increased along with the increasing concentration of 5-azaC. Further analysis found, under the treatment of 5-azaC, plant height and leaf length were both lower than the control, still increased along with the growth of R. glutinosa, however phenotype of R. glutinosa could not appear significant change along with the increase of 5-azaC concentration. Thus, under the reduction of DNA methylation, R. glutinosa appeared obvious dwarfing phenomenon with smaller leaf, indicating moderate demethylation could inhibit growth and development of R. glutinosa, which might be closely related to the status of genomic DNA methylation. (c) 2018 Friends Science Publishers