QTLs detection and pyramiding for stigma exsertion rate in wild rice species by using the single-segment substitution lines
QTLs detection and pyramiding for stigma exsertion rate in wild rice species by using the single-segment substitution lines
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DOI：
10.1007/s11032-020-01157-1
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发表时间：
2020-07
影响因子：
3.1
通讯作者：
Tuo Zou;Hanwei Zhao;Xiaohui Li;Mingmin Zheng;Shaodi Zhang;Lingling Sun;N. He;Xiao-lei Pan;Ziqiang Liu;Xuelin Fu
中科院分区：
文献类型：
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作者：
Tuo Zou;Hanwei Zhao;Xiaohui Li;Mingmin Zheng;Shaodi Zhang;Lingling Sun;N. He;Xiao-lei Pan;Ziqiang Liu;Xuelin Fu
种子产量是制约杂交水稻生产的主要因素之一。雄性不育系柱头外露率（SER）的提高是杂交水稻高产的关键。野生稻是柱头外露的珍贵种质。值得在野生稻品种中挖掘SER相关基因，并探索在水稻育种中利用有益基因的途径。在本研究中，我们利用3个aa -基因组野生稻品种的单片段代换系（SSSLs），共鉴定出分布在11条染色体上的36个稳定的SER qtl。barthii, O。南方猛犸,安藤。稻。2016-2017年，这些qtl的平均遗传效应为14.50% ~ 41.25%。与已报道的qtl进行染色体间隔比较，发现12个qtl为新qtl。此外，在BAR-SSSLs和MER-SSSLs中建立了7个纯合的2-QTL锥体系（PLs），以检测在相同背景下HJX74的QTL相互作用。在7对qSERb3-1/qSERb6-1、qSERb3-1/qSERb8-1和qSERb3-1/qSERb12-1中，3对qSERb3-1/qSERb12-1的上位性效应为正，2018-2019年平均上位性效应分别为13.56%、4.76%和7.29%。B6/B19 （qSERb3-1/qSERb12-1）的平均SER最高，为80.59%，金字塔效应最大，为55.51%。本研究结果表明，SSSLs是野生稻SER QTL检测的优良材料，可以在SSSLs中开发出优良的PLs，用于杂交水稻SER改良育种。
Seed yield is one of the main limitations in hybrid rice production. The improvement of stigma exsertion rate (SER) of male sterility lines is essential for the higher seed yield in hybrid rice. Wild rice species are valuable germplasms with exserted stigma. It is worthy of mining-related genes for SER in wild rice species, as well as probing the way to use the beneficial genes in rice breeding. In the present study, we identified a total number of 36 stable QTLs for the SER distributing on 11 chromosomes by using a set of single-segment substitution lines (SSSLs) of 3 AA-genome wild rice species,O. barthii,O. meridionalis, andO. rufipogon. The mean genetic effects of these QTLs were ranged from 14.50 to 41.25% during 2016–2017. Twelve QTLs among them were novel by comparing the chromosome intervals with the reported QTLs. Besides, 7 homozygous 2-QTL pyramiding lines (PLs) were developed in BAR-SSSLs and MER-SSSLs to detect QTL interactions on the same background of HJX74. The epistatic effects in 3 out of 7 QTL pairs were positive, includingqSERb3-1/qSERb6-1,qSERb3-1/qSERb8-1, andqSERb3-1/qSERb12-1, which mean epistatic effect was 13.56%, 4.76%, and 7.29% in 2018–2019, respectively. The PL of B6/B19 (qSERb3-1/qSERb12-1) had the highest mean SER of 80.59% and the largest pyramiding effect of 55.51%. The results in the present study demonstrated that SSSLs were the elite materials for SER QTL detection from wild rice species, and elite PLs could be developed among SSSLs for SER improvement in hybrid rice breeding.