Molecular Characterization by Using Next-Generation Sequencing of Plasmids Containing blaNDM-7 in Enterobacteriaceae from Calgary, Canada
Molecular Characterization by Using Next-Generation Sequencing of Plasmids Containing blaNDM-7 in Enterobacteriaceae from Calgary, Canada
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DOI：
10.1128/aac.02661-15
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发表时间：
2016-03-01
影响因子：
4.9
通讯作者：
Pitout, J. D.
中科院分区：
文献类型：
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作者：
Chen, L.;Peirano, G.;Pitout, J. D.
带有blaNDM-7的肠杆菌科细菌相对较少见，以前在欧洲、印度、美国和日本已经被描述过。本研究描述了2013-2014年从加拿大卡尔加里4名患者身上分离到的肠杆菌科细菌(肺炎克雷伯氏菌[2]，大肠杆菌[2]，粘质沙雷氏菌[1]，霍尔马切氏肠杆菌[1])的特征。含有blaNDM-7的46,161个碱基的IncX3与其他含有blaNDM的IncX3质粒高度相似，有趣的是，在不同的分离物中显示了相同的结构。这一发现可能表明我们的卫生区域内存在水平传播，也可能表明与医院内流行地区的个人有过接触。感染或定居了含有blaNDM-7 IncX3质粒的细菌的患者会带来感染控制方面的挑战。需要进行流行病学和分子研究，以更好地了解传播动态、危险因素和携带blaNDM-7的细菌的宿主。据我们所知，这是粘质链球菌和霍尔马切氏菌BLA(NDM-7)的首次报道。
Enterobacteriaceae with blaNDM-7 are relatively uncommon and had previously been described in Europe, India, the United States, and Japan. This study describes the characteristics of Enterobacteriaceae (Klebsiella pneumoniae [n = 2], Escherichia coli [n = 2], Serratia marcescens [n = 1], and Enterobacter hormaechei [n = 1] isolates) with blaNDM-7 obtained from 4 patients from Calgary, Canada, from 2013 to 2014. The 46,161-bp IncX3 plasmids with blaNDM-7 are highly similar to other blaNDM-harboring IncX3 plasmids and, interestingly, showed identical structures within the different isolates. This finding may indicate horizontal transmission within our health region, or it may indicate contact with individuals from areas of endemicity within the hospital setting. Patients infected or colonized with bacteria containing blaNDM-7 IncX3 plasmids generate infection control challenges. Epidemiological and molecular studies are required to better understand the dynamics of transmission, the risk factors, and the reservoirs for bacteria harboring blaNDM-7. To the best of our knowledge, this is the first report of S. marcescens and E. hormaechei with bla(NDM-7).