Polyomavirus BK and JC in individuals with chronic kidney failure, kidney transplantation, and healthy controls
Polyomavirus BK and JC in individuals with chronic kidney failure, kidney transplantation, and healthy controls
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DOI：
10.1016/j.jcv.2017.02.003
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发表时间：
2017-04-01
影响因子：
8.8
通讯作者：
Gallottini, Marina
中科院分区：
文献类型：
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作者：
Castroa, Talita;Domingues Fink, Maria Cristina;Gallottini, Marina
背景：通过使用口腔液体，已经采用了诊断和监测全身性疾病个体的新临床方法。多瘤病毒BK（BKPyV）和JC（JCPyV）无症状地感染全球约80%的一般人群，在体内保持潜伏。目的：本研究的目的是检测和定量慢性肾衰竭（CKF）、肾移植（KT）和对照个体的口腔液中的BKPyV和JCPyV，并与传统上用于本试验的血液和尿液中的检测进行比较。CKF组14例，KT组12例，健康对照组20例。结果：第1组所有受试者至少有1份标本BKPyV阳性，14%的受试者JCPyV阳性。第2组BKPyV阳性率为91.7%，JCPyV阳性率为51.7%。在第3组的受试者中，80%为BKPyV阳性，45%为JCPyV阳性。结论：口服液表现出BKPyV和JCPyV的高患病率，与尿液和血液相比同样有效。使用口腔液来检测这些多瘤病毒增强了筛查的阳性率，即使在没有病毒血症的情况下，特别是在不能排尿的个体中。(C)2017爱思唯尔B.V.保留所有权利。
Background: New clinical approaches to diagnose and monitor individuals with systemic diseases have been employed through the use of oral fluids. Polyomavirus BK (BKPyV) and JC (JCPyV) infect asymptomatically around 80% of general population worldwide remaining latent in the body. In case of immunosuppression, a replication can occur, leading to diseases.Objective: The aim of this study was to detect and quantify BKPyV and JCPyV in oral fluids of individuals with chronic kidney failure (CKF), kidney transplantation (KT) and controls compared with their detection in blood and urine, traditionally used for this test.Study design: Forty six subjects were included and distributed into 3 groups: 14 with CKF (Group 1), 12 with KT (Group 2) and 20 healthy individuals (Group 3). In a total, 315 samples were collected and analyzed through RT-PCR, being 151 of gingival crevicular fluid, 46 of saliva, 46 of mouthwash, 43 of blood and 29 of urine.Results: All subjects from group 1 were positive for BKPyV in at least one collected samples and 14% were positive for JCPyV. In Group 2, 91.7% were positive for BKPyV and 51.7% for JCPyV. Among subjects of Group 3, 80% were positive for BKPyV and 45% for JCPyV.Conclusions: Oral fluids exhibited high prevalence of BKPyV and JCPyV and were equally efficient compared to urine and blood. The use of oral fluids to detect these polyomaviruses enhances positivity in screening, even in cases of absence of viremia and especially in individuals who are not able to urinate. (C) 2017 Elsevier B.V. All rights reserved.