The evolutionary history of Cytochrome P450 genes in four filamentous Ascomycetes
The evolutionary history of Cytochrome P450 genes in four filamentous Ascomycetes
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DOI：
10.1186/1471-2148-7-30
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发表时间：
2007-02-26
影响因子：
3.4
通讯作者：
Dean, Ralph A.
中科院分区：
文献类型：
--
作者：
Deng, Jixin;Carbone, Ignazio;Dean, Ralph A.
背景：细胞色素P450系统在真菌适应新生态位的进化过程中起着重要作用。为了阐明细胞色素P450基因在不同生活方式真菌中的进化过程，我们研究了四种丝状子囊菌基因组中的基因获得和丢失模式，其中包括两种腐养菌（构巢曲霉（AN）和粗糙脉孢菌（NC））和两种植物病原体结果：共获得376个P450基因，根据标准命名法将其归属于168个家族。平均而言，每个基因组中每个家族只有1到2个基因。为了解决不同的聚类分析和标准的家庭指定之间的冲突的结果，制定了更高的顺序关系。376个基因被聚为115个家族。随后，一种新的方法的基础上简约的进化模型的建立。基于这些分析，定义了30个不同家族的P450的核心。核心氏族经历了收缩，在所有四个真菌谱系，而新的氏族扩大在所有的NC除外。与其他真菌相比，MG经历了更多的基因和家族增益。结论：4种真菌的P450基因组在编码序列、内含子-外显子结构和基因组位置等方面表现出前所未有的多样性，表明丝状子囊菌中P450基因具有复杂的进化历史。氏族分类和一种新的策略被开发出来研究进化史。核心宗族的收缩和新宗族的扩张。例外的是NC谱系，其表现出纯P450基因丢失。
Background: The Cytochrome P450 system is important in fungal evolution for adapting to novel ecological niches. To elucidate the evolutionary process of cytochrome P450 genes in fungi with different life styles, we studied the patterns of gene gains and losses in the genomes of four filamentous Ascomycetes, including two saprotrophs (Aspergillus nidulans (AN) and Neurospora crassa (NC)) and two plant pathogens (Fusarium graminearum (FG) and Magnaporthe grisea (MG)).Results: A total of 376 P450 genes were assigned to 168 families according to standard nomenclature. On average, only 1 to 2 genes per family were in each genome. To resolve conflicting results between different clustering analyses and standard family designation, a higher order relationship was formulated. 376 genes were clustered into 115 clans. Subsequently a novel approach based on parsimony was developed to build the evolutionary models. Based on these analyses, a core of 30 distinct clans of P450s was defined. The core clans experienced contraction in all four fungal lineages while new clans expanded in all with exception of NC. MG experienced more genes and clans gains compared to the other fungi. Parsimonious analyses unanimously supported one species topology for the four fungi.Conclusion: The four studied fungi exhibit unprecedented diversity in their P450omes in terms of coding sequence, intron-exon structures and genome locations, suggesting a complicated evolutionary history of P450s in filamentous Ascomycetes. Clan classification and a novel strategy were developed to study evolutionary history. Contraction of core clans and expansion of novel clans were identified. The exception was the NC lineage, which exhibited pure P450 gene loss.