Age-related morphological changes in the basement membrane in the stria vascularis of C57BL/6 mice.
Age-related morphological changes in the basement membrane in the stria vascularis of C57BL/6 mice.
复制标题
C57BL/6 小鼠血管纹基底膜与年龄相关的形态变化。
DOI：
10.1007/s00405-014-3478-4
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Yamasoba T.
中科院分区：
文献类型：
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作者：
Suzuki M;Sakamoto T;Kashio A;Yamasoba T.
基底膜阴离子位点（BMAS）参与耳蜗毛细血管中带电大分子的选择性转运。使用阳离子聚乙烯亚胺（PEI），我们研究了年龄相关的变化，在C57 BL/6小鼠耳蜗的BMAS。根据年龄将小鼠分组如下：3天、4周、8周、6个月和12个月。在右侧骨嵴中，使用光学显微镜在石蜡包埋切片中测量血管纹的宽度。将左侧骨软骨浸入0.5%阳离子PEI溶液中并包埋在环氧树脂中。左耳蜗超薄切片进行了检查，使用透射电子显微镜。在4周龄和12月龄小鼠之间观察到血管纹宽度的显著差异。观察PEI在耳蜗毛细血管和上皮基底膜（BM）中的分布。在所有动物中，PEI颗粒均匀分布在螺旋韧带的毛细血管BM和Reissner膜的上皮下BM中。在血管纹中，PEI颗粒在3日龄小鼠的毛细血管BM中均匀分布。在4周龄和8周龄小鼠中，PEI颗粒尺寸明显低于在3日龄小鼠中观察到的那些。在6个月和12个月大的小鼠中，在毛细血管BM中几乎没有检测到PEI颗粒。毛细血管基底膜内、外疏板消失，致密板增大。我们推测C57 BL/6小鼠毛细血管BMAS的增龄性变化可能会影响血管纹中的带电大分子转运系统。
Basement membrane anionic sites (BMAS) are involved in the selective transport of electrically charged macromolecules in cochlear capillaries. Using cationic polyethyleneimine (PEI), we examined age-related changes in BMAS in the cochleae of C57BL/6 mice. The mice were grouped according to age as follows: 3 days, 4 weeks, 8 weeks, 6 months, and 12 months. In the right bony labyrinths, widths of the stria vascularis were measured in paraffin-embedded sections using light microscopy. The left bony labyrinths were immersed in a 0.5 % cationic PEI solution and embedded in epoxy resin. Ultrathin sections of the left cochlea were examined using transmission electron microscopy. A significant difference in stria vascularis width was observed between the 4-week-old and 12-month-old mice. The PEI distribution in the capillary and epithelial basement membranes (BMs) of the cochlea was observed. In all animals, PEI particles were evenly distributed in the capillary BM of the spiral ligament and in the subepithelial BM of Reissner’s membrane. In the stria vascularis, PEI particles were evenly distributed in the capillary BM in 3-day-old mice. In 4- and 8-week-old mice, PEI particle sizes were markedly lower than those observed in 3-day-old mice. In 6- and 12-month-old mice, PEI particles were hardly detected in the strial capillary BM. In the strial capillary BM in these mice, the laminae rarae externa and interna disappeared, but the lamina densa became larger. We speculated that age-related changes of strial capillary BMAS may affect electrically charged macromolecule transport systems in the stria vascularis of C57BL/6 mice.