SWARMER CELL-DIFFERENTIATION OF PROTEUS-MIRABILIS IN FLUID MEDIA
SWARMER CELL-DIFFERENTIATION OF PROTEUS-MIRABILIS IN FLUID MEDIA
复制标题
DOI：
10.1139/m85-196
复制
发表时间：
1985-11-01
影响因子：
2.8
通讯作者：
WALMSLEY, S
中科院分区：
文献类型：
--
作者：
DICK, H;MURRAY, RGE;WALMSLEY, S
在脑心输液等富含液体的介质中培养3-4h后，正常短杆可活动的无隔细丝形成，约占奇异变形杆菌PM23细胞量的80%。在该物种的其他九个代表中，没有观察到这种发育模式。这些细丝被认为相当于在琼脂上形成的沼泽细胞，因为这些细胞停止了翻滚(即趋化性被抑制)，它们形成了大量的鞭毛(即鞭毛的合成和插入被解压)，并且细丝中的核的分布表明存在正常的分离。在添加氨基酸和烟酸的最低限度的培养基中生长的细胞群只由具有滚动运动的短细胞组成，尽管产生了长细胞，并聚集在用普通琼脂固化的相同的培养基上(精琼脂无效)。这些短细胞在37度脑心输液中1~1.5h内分化。C在最初的除法之后。分化的启动条件是良好的基础营养、适宜的阳离子(可能是Ca~(2+)和Na~+，或K~+)，以及粗琼脂和酵母膏中存在的未知的热稳定性有机因素(分子量和lt；10000)。如果启动，培养基中的其他成分会促进羊群分化，这些成分包括有机酸(乳酸)、氨基酸(脯氨酸或丝氨酸)、磷酸盐和适当的离子环境。脑心输注肉汤培养中观察到的长度级序列与计算机生成的生长模型的比较表明，在生长开始时，每个短细胞分裂的50%的产物停止分裂，但在大约5个倍增期内生长，然后从两端以更快的速度(每小时3-5个计时器)分裂细胞，以返回短细胞库。
After 3-4 h in a rich fluid medium such as brain-heart infusion broth, motile nonseptate filaments developed from normal short rods and formed about 80% of the cell mass of Proteus mirabilis PM23. This developmental pattern was not observed in any of the other nine representatives of the species. These filaments were considered to be equivalent to swarmer cells formed on agar media because these cells ceased tumbling (i.e., chemotaxis was repressed), they developed large numbers of flagella (i.e., flagella synthesis and insertion was derepressed), and the distribution of nuclei in the filaments indicated that there was normal segregation. The population of cells grown in a minimal medium supplemented with amino acids and nicotinic acid consisted only of short cells with tumbling motility, despite the production of long cells and swarming on the same medium solidified with ordinary agar (refined agar was not effective). These short cells differentiated in 1-1.5 h in brain-heart infusion broth at 37.degree. C after an initial division. The requirements for initiation of differentiation were good basal nutrition, suitable cations (probably Ca2+ and Na+, or K+), and unknown heat-stable organic factors (molecular weight < 10000) present in crude agar and yeast extract. Other components of media promoted swarmer differentiation if it was initiated and these included organic acid (lactate), amino acids (proline or serine), phosphate, and an appropriate ionic environment. Comparision of the observed sequence of length classes in brain-heart infusion broth culture with computer generated growth models suggested that, at the onset of growth, 50% of the products of each short cell division ceased septation but grew in length for about five doubling periods and then divided cells from each end at a faster rate (3-5 timer per hour) for return to the short cell pool.