Thermosensitive chitosan-Pluronic hydrogel as an injectable cell delivery carrier for cartilage regeneration
Thermosensitive chitosan-Pluronic hydrogel as an injectable cell delivery carrier for cartilage regeneration
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DOI：
10.1016/j.actbio.2009.01.040
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发表时间：
2009-07-01
影响因子：
9.7
通讯作者：
Park, Ki Dong
中科院分区：
文献类型：
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作者：
Park, Kyung Min;Lee, Sang Young;Park, Ki Dong
可注射水凝胶已被研究用于关节软骨再生的潜在应用。本研究设计了一种热敏壳聚糖- pluronic （CP）水凝胶作为软骨再生的可注射细胞递送载体。采用EDC/NHS化学将Pluronic接枝到壳聚糖上，合成了CP缀合物。通过流变学实验考察了CP水凝胶的溶胶-凝胶相变和力学性能。CP溶液在25℃左右经历了溶胶-凝胶转变，其储存模量(G)接近10(4)Pa，突出了该材料作为可注射软骨再生支架的潜力。随着温度的升高，CP水凝胶迅速形成。用扫描电镜观察干燥后CP水凝胶的形态。用牛软骨细胞进行体外细胞培养。28 d后，牛软骨细胞的增殖和糖胺聚糖的合成量均有所增加。这些结果表明CP水凝胶具有作为软骨再生的可注射细胞递送载体的潜力，可以作为一种新的组织工程生物材料。(C) 2009材料学报Elsevier Ltd.出版。版权所有。
Injectable hydrogels have been studied for potential applications for articular cartilage regeneration. In this study, a thermosensitive chitosan-Pluronic (CP) hydrogel was designed as an injectable cell delivery carrier for cartilage regeneration. The CP conjugate was synthesized by grafting Pluronic onto chitosan using EDC/NHS chemistry. The sol-gel phase transition and mechanical properties of the CP hydrogel were examined by rheological experiments. The CP solution underwent a sol-gel transition around 25 degrees C at which the storage modulus (G) approaches 10(4) Pa, highlighting the potential of this material as an injectable scaffold for cartilage regeneration. The CP hydrogel was formed rapidly by increasing the temperature. The morphology of the dried CP hydrogel was observed by scanning electron microscopy. In vitro cell culture was performed using bovine chondrocytes. The proliferation of bovine chondrocytes and the amount of synthesized glycosaminoglycan increased for 28 days. These results suggested that the CP hydrogel has potential as an injectable cell delivery carrier for cartilage regeneration and could serve as a new biomaterial for tissue engineering. (C) 2009 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.