PURIFICATION AND PROPERTIES OF AN ALPHA-(1-]3) GLUCANOHYDROLASE FROM TRICHODERMA-HARZIANUM
PURIFICATION AND PROPERTIES OF AN ALPHA-(1-]3) GLUCANOHYDROLASE FROM TRICHODERMA-HARZIANUM
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DOI：
10.1177/00220345720510022701
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发表时间：
1972-01-01
影响因子：
7.6
通讯作者：
HALLER, R
中科院分区：
文献类型：
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作者：
GUGGENHEIM, B;HALLER, R
材料和方法哈茨木霉（Trichoderma harzianum）（Rifai 2l）OMZ 779分离自在瑞士布鲁格附近的森林中发现的一块分解的树皮。变形链球菌（Streptococcus mutans）OMZ 176的胞外葡聚糖是一种不溶性的葡聚糖。通过高碘酸盐氧化和还原从天然变聚糖制备改性变聚糖。由葡萄糖基转移酶的混合物产生的来自血链球菌804的不溶性凝胶状葡聚糖是一种礼物。还捐赠了木糖、黑四糖、茯苓多糖、白绢病菌胶、香菇多糖、海带多糖和尼日利亚多糖。根据别处描述的方法制备来自牙菌斑的含有不溶性基质多糖的级分。22种试剂。购买Sephadex G-150和蓝色葡聚糖、t Ampholine，§ pH 5-8和Aquacid IIII。所有其他化学品均为试剂级，所用碳水化合物为D构型。培养方法。将哈茨木霉OMZ 779保持在麦芽琼脂上。对于酶生产，将该菌株在满足以下条件的基础培养基中生长：
Materials and Methods STRAIN.-Trichoderma harzianum (Rifai2l) OMZ 779 was isolated from a piece of decomposing bark found in a forest near Brugg, Switzerland.POLYSACCHARIDES.-Mutan, the insoluble extracellular glucan from Streptococcus mutans OMZ 176, was synthesized from su-crose by a mixture of glucosyltransferases, as reported previously.'9 Modified mutan was prepared from native mutan by periodate oxidation and reduction.'l The insoluble gellike dextran from Streptococcus sanguis 804 produced by a mixture of glucosyltransferases was a gift.* Nigerose, nigerotetraose, pachyman, sclerotium rolfsii gum, pseudonigeran, laminarin, and nigeran also were donated. t Fractions from dental plaque containing insoluble matrix polysaccharides were prepared according to the methods described elsewhere. 22 REAGENTs.-Sephadex G-150 and blue dextran, t Ampholine, § pH 5-8, and Aquacid IIII were purchased. All other chemicals were reagent grade, and carbohydrates used were of the D configuration. CULTURE METHODS.-Trichoderma harzianum OMZ 779 was maintained on malt agar.# For enzyme production, this strain was grown in a basal medium meeting the