Effect of cigarette smoking on human oviductal ciliation and ciliogenesis
Effect of cigarette smoking on human oviductal ciliation and ciliogenesis
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DOI：
10.1016/j.fertnstert.2012.08.041
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发表时间：
2013-01-01
影响因子：
6.7
通讯作者：
Burney, Richard O.
中科院分区：
文献类型：
--
作者：
Pier, Bruce;Kazanjian, Avedis;Burney, Richard O.
目的：探讨香烟烟雾暴露对人输卵管上皮纤毛形成和纤毛形成的影响。设计：利用人体输卵管段进行分子分析。环境：学术医疗中心。患者：20名接受选择性输卵管绝育手术的妇女。干预：使用定量免疫组织化学和Western blot分析比较吸烟者和非吸烟者输卵管段纤毛细胞特异性标志物的表达。采用定量聚合酶链反应和定量免疫组织化学方法比较运动纤毛发生过程中转录因子的表达。主要观察指标：输卵管纤毛和参与纤毛发生的转录因子的表达。结果：两组间调解密度无显著差异。吸烟年数和包年历史与调解的密度无关。纤毛转录因子FOXJ1、RFX2、RFX3在各组间表达一致。结论：很少有研究评估吸烟与人输卵管纤毛上皮之间的关系。吸烟似乎不会导致纤毛密度的数量差异，也不会导致纤毛发生因子的表达。我们的研究结果表明，除调解外，病理生理机制是吸烟妇女宫外孕风险增加的原因。[j] .中国农业科学（英文版），2013;39(1):1 - 4。(C) 2013年由美国生殖医学学会出版。)
Objective: To investigate the effect of cigarette smoke exposure on ciliation and ciliogenesis in human oviductal epithelium.Design: Molecular analysis using human tubal segments.Setting: Academic medical center.Patient(s): Twenty women undergoing elective tubal sterilization procedure.Intervention(s): Expression of ciliated cell-specific markers was compared in tubal segments from smokers and nonsmokers using quantitative immunohistochemistry and Western blot analysis. The expression of transcription factors in the motile ciliogenesis program was compared using quantitative polymerase chain reaction and quantitative immunohistochemistry.Main Outcome Measure(s): Oviductal ciliation and expression of transcription factors involved in ciliogenesis.Result(s): No significant differences were detected in density of ciliation between groups. Neither number of years of smoking nor pack-year history correlated with density of ciliation. Expression of ciliogenic transcription factors FOXJ1, RFX2, and RFX3 was consistent between groups.Conclusion(s): Few studies have evaluated the relationship between smoking and ciliated epithelium in human oviducts. Cigarette smoking does not seem to result in quantitative differences in the density of ciliation nor expression of ciliogenesis factors. Our findings suggest that pathophysiologic mechanisms other than ciliation account for the increased risk of ectopic pregnancy in women who smoke. (Fertil Steril (R) 2013;99:199-205. (C) 2013 by American Society for Reproductive Medicine.)