IL-1-BETA AND IL-6 INDUCE HYPERPLASIA AND HYPERTROPHY OF CULTURED GUINEA-PIG AIRWAY SMOOTH-MUSCLE CELLS
IL-1-BETA AND IL-6 INDUCE HYPERPLASIA AND HYPERTROPHY OF CULTURED GUINEA-PIG AIRWAY SMOOTH-MUSCLE CELLS
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DOI：
10.1152/jappl.1995.78.4.1555
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发表时间：
1995-04-01
影响因子：
3.3
通讯作者：
SOUHRADA, M
中科院分区：
文献类型：
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作者：
DE, S;ZELAZNY, ET;SOUHRADA, M
将豚鼠气道平滑肌（ASM）细胞维持在原代组织培养物中（第1-3代）。在吲哚美辛（1 μ g/ml）存在下，将细胞暴露于人重组白细胞介素-1 β（IL-1 β; 20-100 pg/ml）或白细胞介素-6（IL-6; 1-4 ng/ml）长达5天。ASM细胞的增殖用两种技术评估，用血细胞计数器直接计数细胞和[H-3]胸苷掺入校正总蛋白含量。通过[H-3]亮氨酸掺入（蛋白质合成的评价）、测定总DNA含量、每个细胞的DNA含量和每个细胞的蛋白质含量来评估ASM细胞的肥大。我们观察到，ASM细胞暴露于所有研究浓度的人重组IL-1 β或IL-6，显著增加了细胞数量以及[H-3]胸苷掺入ASM细胞。我们还发现，暴露于这两种细胞因子的ASM增加[H-3]亮氨酸掺入到ASM细胞中，并增加每个细胞的蛋白质含量和DNA含量。这些变化也具有浓度依赖性。我们的结论是，两个促炎细胞因子，IL-1 β和IL-6，这是目前在哮喘肺，增加了ASM细胞的增殖（增生），以及他们的整体大小和大小的核，作为测量的生化标志物。这些发现与ASM肥大的存在相一致。
Guinea pig airway smooth muscle (ASM) cells were maintained in a primary tissue culture (passages 1-3). Cells were exposed to human recombinant interleukin-1 beta (IL-1 beta; 20-100 pg/ml) or interleukin-6 (IL-6; 1-4 ng/ml) in the presence of indomethacin (1 mu g/ml) for up to 5 days. Proliferation of ASM cells was assessed with two techniques, direct counting of cells with a hemacytometer and [H-3]thymidine incorporation corrected for total protein content. Hypertrophy of ASM cells was assessed by [H-3]leucine incorporation (evaluation of protein synthesis), determination of total DNA content, DNA content per cell, and protein content per cell. We observed that the exposure of ASM cells to human recombinant IL-1 beta or IL-6, in all studied concentrations, significantly increased the number of cells as well as [H-3]thymidine incorporation into ASM cells. We also found that exposure of ASM to these two cytokines increased [H-3]leucine incorporation into the ASM cells and increased protein content and DNA content per single cell. These changes were also concentration dependent. We conclude that the two proinflammatory cytokines, IL-1 beta and IL-6, which are present in asthmatic lungs, increased the proliferation of ASM cells (hyperplasia) as well as their overall size and size of their nuclei, as measured by biochemical markers. These findings are compatible with the presence of ASM hypertrophy.