Manipulation-specific cortical activity as mice handle food.
Manipulation-specific cortical activity as mice handle food.
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DOI：
10.1016/j.cub.2022.09.045
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发表时间：
2022-11-21
期刊：
影响因子：
9.2
通讯作者：
Shepherd, Gordon M G
中科院分区：
文献类型：
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作者：
Barrett, John M;Martin, Megan E;Shepherd, Gordon M G
食物处理提供了独特但在很大程度上未被探索的机会，以一种自然的、在行为学上必不可少的、运动学丰富的手动灵巧性形式，研究皮质活动如何与前肢运动相关。为了确定这些关系，我们记录了小鼠处理食物时的高速(1000fps)视频和多通道电生理皮层棘波活动。视频的高时间分辨率使我们能够将主动操作(口腔)事件分解为特征子运动，从而能够对大脑皮层活动进行事件对齐分析。在处理食物的过程中，前肢M1的活动受到强烈的调节，通常在口腔活动期间较高，在保持间隔期间较低。光发生沉默和刺激前肢M1神经元部分影响食物处理运动，分别发挥抑制和激活效应。我们还将分析扩展到前肢S1和侧肢M1，发现所有三个区域的口腔相关活动大致相似。然而，每个区域的活动呈现出不同的时间和相位/紧张的时间分布，通过非负矩阵因式分解的进一步分析表明，这归因于活动类别的区域特定组成。根据这三个区域的活动可以准确地预测当前或未来的前肢位置，这表明这些区域的皮质活动包含关于食物处理过程中前肢运动的高信息量。因此，这些结果表明，在食物处理过程中，皮质活动是特定于操作的，分布在多个感觉运动区，并且大致相似，同时也显示出特定于区域和亚运动的关系，具有这种复杂的、自由对象处理的手动灵巧性的自然形式的快速运动学特征。Barrett等人。使用高速视频和多电极记录来研究食物处理的神经基础，这是啮齿类动物、灵长类动物和其他手工灵巧的动物的一种在行为学上至关重要的前肢行为。在灵巧的口腔操作过程中，小鼠新皮质的多个区域显示出编码前肢位置的活动变化。
Food-handling offers unique yet largely unexplored opportunities to investigate how cortical activity relates to forelimb movements in a natural, ethologically essential, and kinematically rich form of manual dexterity. To determine these relationships, we recorded high speed (1000 fps) video and multi-channel electrophysiological cortical spiking activity while mice handled food. The high temporal resolution of the video allowed us to decompose active manipulation (‘oromanual’) events into characteristic submovements, enabling event-aligned analysis of cortical activity. Activity in forelimb M1 was strongly modulated during food-handling, generally higher during oromanual events and lower during holding intervals. Optogenetic silencing and stimulation of forelimb M1 neurons partially affected food-handling movements, exerting suppressive and activating effects, respectively. We also extended the analysis to forelimb S1 and lateral M1, finding broadly similar oromanual-related activity across all three areas. However, each area’s activity displayed a distinct timing and phasic/tonic temporal profile, which further analysis by non-negative matrix factorization demonstrated to be attributable to area-specific composition of activity classes. Current or future forelimb position could be accurately predicted from activity in all three regions, indicating that the cortical activity in these areas contains high information content about forelimb movements during food-handling. These results thus establish that cortical activity during food-handling is manipulation-specific, distributed, and broadly similar across multiple sensorimotor areas, while also exhibiting area- and submovement-specific relationships with the fast kinematic hallmarks of this natural form of complex, free-object-handling manual dexterity. Barrett et al. use high-speed video and multi-electrode recordings to investigate the neural basis of food-handling, an ethologically critical forelimb behavior in rodents, primates, and other manually dexterous animals. Multiple areas of mouse neocortex show activity changes that encode forelimb position during dexterous oromanual manipulation.