Comparative efficacy of surface drying and re-drying seed priming in rice: changes in emergence, seedling growth and associated metabolic events
Comparative efficacy of surface drying and re-drying seed priming in rice: changes in emergence, seedling growth and associated metabolic events
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DOI：
10.1007/s10333-009-0170-1
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发表时间：
2010-03-01
影响因子：
2.2
通讯作者：
Asad, Saeed A.
中科院分区：
文献类型：
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作者：
Farooq, Muhammad;Wahid, Abdul;Asad, Saeed A.
在种子引发过程中，种子被浸泡在低水势的溶液中，然后干燥到原来的重量。本研究旨在评估不同种子引发处理的比较表现，并探讨再干燥是否必要？细粒香稻(Oryza sativa L.)以超级巴斯马蒂为试材，分别用CaCl2和KCl2(psi(S)-1.25 Mpa)进行渗透硬化，每隔1个周期进行24 h的渗透硬化处理，再用加氢引发和抗坏血酸引发(10 mg L~(-1))处理48 h。在每次处理后的一组中，用蒸馏水对种子进行三次表面清洗，然后用强迫空气将种子烘干到接近原始水分的水平。在另一种情况下，用蒸馏水清洗表面后，用吸墨纸将种子表面干燥并立即播种。所有引种处理均能促进出苗、幼苗生长和贮藏期代谢。表面干燥对水稻种子活力的促进更有效，这从早出苗和同步出苗中可见一斑。此外，尽管发芽率和叶片评分相似，但两种处理的地上部和根长、幼苗干重、根系评分、α-淀粉酶活性、可溶性糖和脱氢酶活性也得到了提高。结果表明，表面干燥比接近原始重量的复干更有效，而CaCl2渗透硬化效果最好。提高水稻产量和合理利用水资源是当务之急，旱作水稻是一个有吸引力的替代方案。然而，摊位设置不佳和不稳定是其广泛采用的主要障碍之一。在这方面，种子引发技术是在新水稻栽培中实现适当立地的实用方法。它们有助于在最佳和不利的土壤条件下提高单位面积的秧苗密度，并可用于改善旱稻的表现。
In seed priming, seeds are soaked in a solution of low water potential and dried back to their original weight. This study was conducted to evaluate the comparative performance of different seed priming treatments and to investigate whether re-drying is essential or not? Seeds of fine grain aromatic rice (Oryza sativa L.) cultivar Super-Basmati were subjected to hardening, osmohardening with CaCl2 and KCl (psi (s) -1.25 MPa) each for 24 h (one cycle), and hydropriming and ascorbate priming (10 mg L-1) for 48 h. Seeds were primed in two sets. In one set after each treatment, seeds were given three surface washings with distilled water and dried back near to original moisture contents with forced air. In the other case, after surface washings with distilled water, seeds were surface dried using blotting paper and sown immediately. All the priming treatments improved the emergence, seedling growth and reserve metabolism. Use of surface drying was more effective for rice seed invigoration, as evident from earlier and synchronized seedling emergence. Moreover, shoot and root length, seedling dry weight, root score, alpha-amylase activity, soluble sugars and dehydrogenase activity were also improved, although germination percentage and leaf score were similar from both strategies. Results suggested that surface drying, rather than re-drying close to original weight, was more effective, while among the treatments, osmohardening with CaCl2 was the most effective. Increasing rice production with judicious use of water is need of the day, and aerobic rice cultivation is an attractive alternative for this purpose. However, poor and erratic stand establishment is one of the major hindrances in its wide scale adoption. In this regard, seed priming techniques are pragmatic approaches to achieve proper stand establishment in the new rice culture. They help in improving seedling density per unit area under optimal and adverse soil conditions and may be opted to improve the performance of aerobic rice.