The adsorption of Pseudomonas aeruginosa bacteriophage φKMV is dependent on expression regulation of type IV pili genes
The adsorption of Pseudomonas aeruginosa bacteriophage φKMV is dependent on expression regulation of type IV pili genes
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DOI：
10.1111/j.1574-6968.2009.01640.x
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发表时间：
2009-07-01
影响因子：
2.1
通讯作者：
Lavigne, Rob
中科院分区：
文献类型：
--
作者：
Chibeu, Andrew;Ceyssens, Pieter-Jan;Lavigne, Rob
铜绿假单胞菌噬菌体phi KMV需要IV型皮利进行感染，如从噬菌体感染抗性宿主菌株突变体的表型表征和噬菌体吸附测定所观察到的。产生宿主（铜绿假单胞菌PAO 1）基因组DNA的粘粒克隆文库，并用于选择能够在抗性突变体中恢复phi KMV感染的克隆。这种互补粘粒也重新建立了IV型纤毛依赖性抽搐运动。铜绿假单胞菌PAO 1 Delta pilA突变体对噬菌体的抗性证实了噬菌体phi KMV感染性和IV型皮利之间的相关性，沿着其相关的抽搐运动性。补充粘粒的亚克隆和进一步的噬菌体感染分析和运动性测定表明，ponA和pilMNOPQ基因产物之间的共同调控机制和/或相互作用是噬菌体phi KMV感染性所必需的。
Pseudomonas aeruginosa bacteriophage phi KMV requires type IV pili for infection, as observed from the phenotypic characterization and phage adsorption assays on a phage infection-resistant host strain mutant. A cosmid clone library of the host (P. aeruginosa PAO1) genomic DNA was generated and used to select for a clone that was able to restore phi KMV infection in the resistant mutant. This complementing cosmid also re-established type IV pili-dependent twitching motility. The correlation between bacteriophage phi KMV infectivity and type IV pili, along with its associated twitching motility, was confirmed by the resistance of a P. aeruginosa PAO1 Delta pilA mutant to the phage. Subcloning of the complementing cosmid and further phage infection analysis and motility assays suggests that a common regulatory mechanism and/or interaction between the ponA and pilMNOPQ gene products are essential for bacteriophage phi KMV infectivity.